Rapid and sensitive detection of viral particles by coupling redox cycling and electrophoretic enrichment.
Rapid and sensitive detection of viral particles by coupling redox cycling and electrophoretic enrichment.
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DOI：
10.1016/j.bios.2022.114198
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发表时间：
2022-07-15
影响因子：
12.6
通讯作者：
Ebrahimi A
中科院分区：
文献类型：
--
作者：
Butler D;Ebrahimi A
COVID-19疫情凸显了对快速、低成本和敏感的病毒检测平台的需求，以监测和缓解大范围疫情。电化学传感器是填补这一角色的可行选择，但仍然需要改善信号幅度，特别是对于早期检测和低病毒载量。在此，有限元分析的一种新的生物传感器的概念，用于单病毒粒子计数使用的发生器-收集器微电极设计。所提出的设计结合了氧化还原循环放大的电化学电流与荧光素驱动的电极-粒子碰撞，用于快速病毒检测。实验（如扫描速率，集电极偏置）和几何因素的影响进行了研究，以优化传感器的设计。两个发生器-收集器配置进行了探讨：一个环-盘配置，以分析固着液滴和一个叉指电极（IDE）的设计容纳在一个微通道。对于环盘配置，我们计算出的放大因子为1.55和收集器效率为1.00.8的生成器-收集器间距为600 nm。对于IDE，收集器效率甚至更大，接近1。双电极模式对于增加电流和电场强度至关重要。因此，与单一模式相比，病毒捕获时的当前步骤要大一个数量级以上。此外，单个病毒捕获时间从700多秒缩短至20秒。总体而言，病毒捕获的频率和电化学电流阶跃的幅度取决于病毒性质和电极配置，由于微通道中更好的颗粒限制，IDE能够在几秒钟内检测单个病毒。
The COVID-19 pandemic has highlighted the need for rapid, low-cost, and sensitive virus detection platforms to monitor and mitigate widespread outbreaks. Electrochemical sensors are a viable choice to fill this role but still require improvements to the signal magnitude, especially for early detection and low viral loads. Herein, finite element analysis of a novel biosensor concept for single virion counting using a generator-collector microelectrode design is presented. The proposed design combines a redox-cycling amplified electrochemical current with electrophoresis-driven electrode-particle collision for rapid virus detection. The effects of experimental (e.g. scan rate, collector bias) and geometric factors are studied to optimize the sensor design. Two generator-collector configurations are explored: a ring-disk configuration to analyze sessile droplets and an interdigitated electrode (IDE) design housed in a microchannel. For the ring-disk configuration, we calculate an amplification factor of ∼5 and collector efficiency of ∼0.8 for a generator-collector spacing of 600 nm. For the IDE, the collector efficiency is even larger, approaching unity. The dual-electrode mode is critical for increasing the current and electric field strength. As a result, the current steps upon virus capture are more than an order of magnitude larger compared to single-mode. Additionally, single virus capture times are reduced from over 700 s down to ∼20 s. Overall, the frequency of virus capture and magnitude of the electrochemical current steps depend on the virus properties and electrode configuration, with the IDE capable of single virus detection within seconds owing to better particle confinement in the microchannel.
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