The effects of age, exposure history and malaria infection on the susceptibility of Anopheles mosquitoes to low concentrations of pyrethroid.
The effects of age, exposure history and malaria infection on the susceptibility of Anopheles mosquitoes to low concentrations of pyrethroid.
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DOI：
10.1371/journal.pone.0024968
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Read AF
中科院分区：
文献类型：
--
作者：
Glunt KD;Thomas MB;Read AF
化学杀虫剂是疟疾控制方案的关键组成部分。它们消灭大量蚊子的能力使它们能够迅速阻断疾病的传播，但这种致命性也为杀虫剂的抗药性带来了巨大的选择。针对实际负责将疟原虫传播给人类的蚊子种群的一小部分进行控制，将减少抗药性的选择，但保持有效的疟疾控制。在这里，我们要问的是，仅仅降低杀虫剂制剂中活性成分的浓度是否可以优先杀死感染疟疾的蚊子和/或具有潜在传染性的蚊子，即老蚊子。使用修改的WHO耐药性监测试验，我们暴露未感染的斯氏按蚊女性低浓度的拟除虫菊酯氯菊酯在第4，8，12和16天后出现和监测生存至少30天，以评估的直接和长期影响反复暴露的蚊子年龄。我们还暴露了夏氏疟原虫和约氏疟原虫感染的An。stephensi雌性氯菊酯暴露并不总是增加蚊子对随后的杀虫剂暴露的易感性，尽管年龄较大的蚊子更容易受到影响。血餐略有改善杀虫剂暴露后的生存，疟疾感染并没有明显增加杀虫剂的敏感性。在连续的喂养周期中暴露于低浓度大大改变了队列的年龄结构。我们的数据表明，在可以实现高杀虫剂覆盖率的情况下，低浓度制剂有能力减少疾病传播，而不需要通过目前的做法进行大规模的抗性选择。
Chemical insecticides are critical components of malaria control programs. Their ability to eliminate huge numbers of mosquitoes allows them to swiftly interrupt disease transmission, but that lethality also imposes immense selection for insecticide resistance. Targeting control at the small portion of the mosquito population actually responsible for transmitting malaria parasites to humans would reduce selection for resistance, yet maintain effective malaria control. Here, we ask whether simply lowering the concentration of the active ingredient in insecticide formulations could preferentially kill mosquitoes infected with malaria and/or those that are potentially infectious, namely, old mosquitoes. Using modified WHO resistance-monitoring assays, we exposed uninfected Anopheles stephensi females to low concentrations of the pyrethroid permethrin at days 4, 8, 12, and 16 days post-emergence and monitored survival for at least 30 days to evaluate the immediate and long-term effects of repeated exposure as mosquitoes aged. We also exposed Plasmodium chabaudi- and P. yoelii-infected An. stephensi females. Permethrin exposure did not consistently increase mosquito susceptibility to subsequent insecticide exposure, though older mosquitoes were more susceptible. A blood meal slightly improved survival after insecticide exposure; malaria infection did not detectably increase insecticide susceptibility. Exposure to low concentrations over successive feeding cycles substantially altered cohort age-structure. Our data suggest the possibility that, where high insecticide coverage can be achieved, low concentration formulations have the capacity to reduce disease transmission without the massive selection for resistance imposed by current practice.
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影响因子：
1.9
作者：
Hunt, RH;Brooke, BD;Coetzee, M
通讯作者：
Coetzee, M
影响因子：
2.4
作者：
Ferguson, HM;Rivero, A;Read, AF
通讯作者：
Read, AF
影响因子：
2.2
作者：
CURTIS, CF;COOK, LM;WOOD, RJ
通讯作者：
WOOD, RJ
影响因子：
4.1
作者：
Koella JC;Lynch PA;Thomas MB;Read AF
通讯作者：
Read AF
DOI：
10.1098/rspa.2010.0413
发表时间：
2011-08-08
影响因子：
3.5
作者：
Gourley, Stephen A.;Liu, Rongsong;Wu, Jianhong
通讯作者：
Wu, Jianhong