Protocadherin-17 function in Zebrafish retinal development.
Protocadherin-17 function in Zebrafish retinal development.
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DOI：
10.1002/dneu.22053
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发表时间：
2013-04
影响因子：
3
通讯作者：
Liu, Qin
中科院分区：
文献类型：
--
作者：
Chen, Yun;Londraville, Richard;Brickner, Sarah;El-Shaar, Lana;Fankhauser, Kelsee;Dearth, Cassandra;Fulton, Leah;Sochacka, Alicja;Bhattarai, Sunil;Marrs, James A.;Liu, Qin
钙粘蛋白细胞粘附分子在脊椎动物的发育包括视网膜的发育中起着至关重要的作用。大多数研究都集中在研究经典钙粘蛋白（如N-钙粘蛋白）在视网膜发育中的功能。关于原钙粘蛋白在脊椎动物视觉系统发育中的作用的研究还很少。我们以前表明，原钙粘蛋白-17 mRNA表达在视网膜发育的关键阶段，在发展中的斑马鱼视网膜。为了深入了解原钙粘蛋白-17在视网膜形成中的功能，我们分析了注射原钙粘蛋白-17特异性反义吗啉代寡核苷酸（MO）的斑马鱼胚胎中视网膜细胞的眼发育和分化。原钙粘蛋白-17敲除胚胎（pcdh 17变形体）具有显著减少的眼睛，主要是由于细胞增殖减少。几种视网膜细胞类型（例如视网膜神经节细胞）的分化也在pcdh 17变形体中被破坏。通过注射原钙粘蛋白-17 mRNA实现表型拯救。将体内原钙粘蛋白-17 MO注射到胚胎斑马鱼的一只眼睛中导致类似的眼睛缺陷。我们的研究结果表明，原钙粘蛋白-17在斑马鱼视网膜的正常形成中起着重要的作用。
Cadherin cell adhesion molecules play crucial roles in vertebrate development including the development of the retina. Most studies have focused on examining functions of classic cadherins (e.g. N-cadherin) in retinal development. There is little information on the function of protocadherins in the development of the vertebrate visual system. We previously showed that protocadherin-17 mRNA was expressed in developing zebrafish retina during critical stages of the retinal development. To gain insight into protocadherin-17 function in the formation of the retina, we analyzed eye development and differentiation of retinal cells in zebrafish embryos injected with protocadherin-17 specific antisense morpholino oligonucleotides (MOs). Protocadherin-17 knockdown embryos (pcdh17 morphants) had significantly reduced eyes due mainly to decreased cell proliferation. Differentiation of several retinal cell types (e.g. retinal ganglion cells) was also disrupted in the pcdh17 morphants. Phenotypic rescue was achieved by injection of protocadherin-17 mRNA. Injection of a vivo-protocadherin-17 MO into one eye of embryonic zebrafish resulted in similar eye defects. Our results suggest that protocadherin-17 plays an important role in the normal formation of the zebrafish retina.
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DOI：
10.1083/jcb.153.4.865
发表时间：
2001-05-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
Carmena M
影响因子：
1.2
作者：
Gaitan, Yaned;Bouchard, Maxime
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影响因子：
64.5
作者：
Furukawa, T;Morrow, EM;Cepko, CL
通讯作者：
Cepko, CL
DOI：
10.1083/jcb.201007008
发表时间：
2010-11-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Biswas S;Emond MR;Jontes JD
通讯作者：
Jontes JD
影响因子：
2.7
作者：
Emond, Michelle R.;Biswas, Sayantanee;Jontes, James D.
通讯作者：
Jontes, James D.