Amorphous Silica: A New Antioxidant Role for Rapid Critical-Sized Bone Defect Healing.
Amorphous Silica: A New Antioxidant Role for Rapid Critical-Sized Bone Defect Healing.
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DOI：
10.1002/adhm.201600203
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发表时间：
2016-09
影响因子：
10
通讯作者：
Varanasi VG
中科院分区：
文献类型：
--
作者：
Ilyas A;Odatsu T;Shah A;Monte F;Kim HK;Kramer P;Aswath PB;Varanasi VG
由于严重的骨质流失，创伤性骨折会导致结构不稳定。这样的骨折会产生大量的活性氧（ROS），从而导致氧化应激。过多且延长的活性氧活性会阻碍成骨细胞分化，并导致愈合时间延长。刺激抗氧化剂如超氧化物歧化酶（SOD 1），对于减少ROS，刺激骨生成，加强胶原蛋白和矿物质形成至关重要。然而，目前没有固定装置显示出通过抗氧化剂表达增强胶原基质形成的能力。在这里，我们报告等离子体增强化学气相沉积（PECVD）为基础的无定形氮氧化硅（Si（ON）x）作为一种潜在的新的骨折愈合生物材料，粘附在植入物表面，释放Si+4，以增强骨生成，并形成表面羟基磷灰石胶原矿物质附着。这些材料在生理环境中长时间提供Si+4的持续释放。溶解速率部分取决于膜的化学性质，并且可以通过改变O/N比来控制。Si+4的存在增强了SOD 1，其刺激下游的其他成骨标志物并导致快速矿物质形成。使用大鼠临界尺寸颅骨缺损模型的体内测试显示，与对照组相比，这些生物材料的骨再生更快，这意味着所提供的生物材料的临床意义。
Traumatic fractures cause structurally unstable sites due to severe bone loss. Such fractures generate a high yield of reactive oxygen species (ROS) that can lead to oxidative stress. Excessive and prolonged ROS activity impedes osteoblast differentiation, and instigates long healing times. Stimulation of antioxidants such as superoxide dismutase (SOD1), are crucial to reduce ROS, stimulate osteogenesis, and strengthen collagen and mineral formation. Yet, no current fixative devices have shown an ability to enhance collagen matrix formation through antioxidant expression. Here, we report plasma-enhanced chemical vapor deposition (PECVD)-based amorphous silicon oxynitride (Si(ON)x) as a potential new fracture healing biomaterial that adheres well to the implant surface, releases Si+4 to enhance osteogenesis, and forms a surface hydroxyapatite for collagen mineral attachment. These materials provide a sustained release of Si+4 in physiological environment for extended times. The dissolution rate partially depends on the film chemistry and can be controlled by varying O/N ratio. The presence of Si+4 enhances SOD1, which stimulates other osteogenic markers downstream and leads to rapid mineral formation. In-vivo testing using a rat critical-sized calvarial defect model shows a more rapid bone-regeneration for these biomaterials as compared to control groups, that implies the clinical significance of the presented biomaterial.
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