Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice.
Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice.
复制标题
口服益生菌乳酸杆菌调节细胞因子的产生和TLR表达，可改善小鼠肠肠typhimurium typhimurium感染的免疫反应。
DOI：
10.1186/1471-2180-11-177
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发表时间：
2011-08-03
期刊：
影响因子：
4.2
通讯作者：
de Moreno de Leblanc A
中科院分区：
文献类型：
--
作者：
Castillo NA;Perdigón G;de Moreno de Leblanc A
沙门氏菌引起的腹泻感染是发展中国家儿童发病和死亡的主要原因之一。沙门氏菌会引起从轻度胃肠炎到肠热病的各种疾病，这取决于所涉及的血清型、感染剂量、物种、年龄和宿主的免疫状态。益生菌被认为是预防这种病原体感染的一种有吸引力的替代可能性。先前我们证明了在用沙门氏菌鼠伤寒血清型（S.鼠伤寒沙门氏菌）降低了沙门氏菌感染的严重程度。本研究的目的是深入了解这种益生菌如何发挥其作用，通过评估其对肠道免疫应答的诱导和效应位点中促炎（TNFα，IFNγ）和抗炎（IL-10）细胞因子的表达和分泌的影响，并分析健康和感染小鼠中toll样受体（TLR 2，TLR 4，TLR 5和TLR 9）的表达。给予健康小鼠益生菌增加了TLR 2、TLR 4和TLR 9的表达，并改善了肠道免疫应答诱导部位（派伊尔集合淋巴结）中TNFα、IFNγ和IL-10的产生和分泌。感染后，在沙门氏菌攻击前后连续给予益生菌，通过调节炎症反应（主要在肠道的免疫效应部位）、降低TNFα并增加小肠固有层中IFNγ、IL-6和IL-10的产生来保护宿主。口服L. casei CRL 431在用S.鼠伤寒这些变化显示了这种益生菌菌株对S.鼠伤寒，提供了一种替代方法，以减少感染的严重程度。
Diarrheal infections caused by Salmonella, are one of the major causes of childhood morbidity and mortality in developing countries. Salmonella causes various diseases that range from mild gastroenteritis to enteric fever, depending on the serovar involved, infective dose, species, age and immune status of the host. Probiotics are proposed as an attractive alternative possibility in the prevention against this pathogen infection. Previously we demonstrated that continuous Lactobacillus casei CRL 431 administration to BALB/c mice before and after challenge with Salmonella enterica serovar Typhimurium (S. Typhimurium) decreased the severity of Salmonella infection. The aim of the present work was to deep into the knowledge about how this probiotic bacterium exerts its effect, by assessing its impact on the expression and secretion of pro-inflammatory (TNFα, IFNγ) and anti-inflammatory (IL-10) cytokines in the inductor and effector sites of the gut immune response, and analyzing toll-like receptor (TLR2, TLR4, TLR5 and TLR9) expressions in both healthy and infected mice. Probiotic administration to healthy mice increased the expression of TLR2, TLR4 and TLR9 and improved the production and secretion of TNFα, IFNγ and IL-10 in the inductor sites of the gut immune response (Peyer's patches). Post infection, the continuous probiotic administration, before and after Salmonella challenge, protected the host by modulating the inflammatory response, mainly in the immune effector site of the gut, decreasing TNFα and increasing IFNγ, IL-6 and IL-10 production in the lamina propria of the small intestine. The oral administration of L. casei CRL 431 induces variations in the cytokine profile and in the TLRs expression previous and also after the challenge with S. Typhimurium. These changes show some of the immune mechanisms implicated in the protective effect of this probiotic strain against S. Typhimurium, providing an alternative way to reduce the severity of the infection.
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影响因子：
8
作者：
通讯作者：
--
影响因子：
3.8
作者：
Dogi, C. A.;Galdeano, C. Maldonado;Perdigon, Gabriela
通讯作者：
Perdigon, Gabriela
DOI：
10.1016/j.ijfoodmicro.2010.01.020
发表时间：
2010-04-15
影响因子：
5.4
作者：
de Moreno de LeBlanc, Alejandra;Castillo, Natalia A.;Perdigon, Gabriela
通讯作者：
Perdigon, Gabriela
影响因子：
2.8
作者：
Dogi, C. A.;Weill, F.;Perdigon, G.
通讯作者：
Perdigon, G.
DOI：
10.1590/s0074-02762009000200017
发表时间：
2009-03-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Brandão, Geane Peroni;Melo, Maria Norma;Vitor, Ricardo Wagner Almeida
通讯作者：
Vitor, Ricardo Wagner Almeida