Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete.
Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete.
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DOI：
10.1186/s13568-018-0648-6
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发表时间：
2018-07-17
期刊：
影响因子：
3.7
通讯作者：
Jia R
中科院分区：
文献类型：
--
作者：
Duan Z;Shen R;Liu B;Yao M;Jia R
Irpex milkeus F17 以其降解顽固芳香污染物的能力而闻名，这主要是由于它能够产生的锰过氧化物酶 (MnP) 的作用。最近，该菌株的基因组测序和注释提供了木质素分解过氧化物酶基因家族的全面图景。除了揭示 13 MnP 的存在之外，还在 I. milkeus F17 基因组中发现了 5 种染料脱色过氧化物酶 (DyP) 的基因，它们与真菌 II 类过氧化物酶无关。在本研究中，分析了代表两个不同血红素过氧化物酶家族的 5 种 DyP 和 13 种 MnP 的氨基酸序列。其中，DyP (Il-DyP4) 和 MnP (Il-MnP6) 两种酶分别在大肠杆菌中表达，并通过比较它们的分子模型、底物特异性和催化特征来表征。结果表明，Il-DyP4对一些代表性底物具有较高的催化效率，在酸性条件下对多种合成染料具有较强的脱色能力。基于电化学测量，发现 Il-DyP4 在 pH 3.5 时具有 27 mV 的高氧化还原电位，优于 Il-MnP6 (− 75 mV)，从而有助于其氧化高氧化还原电位底物（例如藜芦醇和聚合物染料 Poly R-478）的能力。结果凸显了 Il-DyP4 在工业和环境应用中的潜力。本文的在线版本 (10.1186/s13568-018-0648-6) 包含补充材料，可供授权用户使用。
Irpex lacteus F17 is well-known for its ability to degrade recalcitrant aromatic pollutants, which mainly results from the action of the manganese peroxidase (MnP) that it is able to produce. Recently, the genome sequencing and annotation of this strain provided comprehensive picture of the ligninolytic peroxidase gene family. In addition to revealing the presence of 13 MnPs, genes for five dye-decolorizing peroxidases (DyPs) were also discovered in the I. lacteus F17 genome, which are unrelated to the fungal class II peroxidases. In the present study, amino acid sequences of five DyPs and 13 MnPs, representing two different families of heme peroxidases, were analyzed. Of these, two enzymes, a DyP (Il-DyP4) and a MnP (Il-MnP6) were expressed respectively in Escherichia coli, and were characterized by comparing their molecular models, substrate specificities, and catalytic features. The results showed that Il-DyP4 possessed a higher catalytic efficiency for some representative substrates, and a stronger decolorizing ability to a wide range of synthetic dyes in acidic conditions. Based on electrochemical measurements, Il-DyP4 was found to have a high redox potential of 27 mV at pH 3.5, which was superior to that of Il-MnP6 (− 75 mV), thereby contributing to its ability to oxidize high redox potential substrates, such as veratryl alcohol and polymeric dye Poly R-478. The results highlighted the potential of Il-DyP4 for use in industrial and environmental applications. The online version of this article (10.1186/s13568-018-0648-6) contains supplementary material, which is available to authorized users.
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影响因子：
2.4
作者：
Kinne, Matthias;Poraj-Kobielska, Marzena;Hofrichter, Martin
通讯作者：
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影响因子：
3
作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
Hofrichter M
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DOI：
10.1016/j.saa.2009.11.033
发表时间：
2010-02-01
影响因子：
4.4
作者：
Guo, Shaofen;Wang, Lihong;Huang, Xiaohua
通讯作者：
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影响因子：
10.4
作者：
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