Exploring early steps in biofilm formation: set-up of an experimental system for molecular studies.
Exploring early steps in biofilm formation: set-up of an experimental system for molecular studies.
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DOI：
10.1186/s12866-014-0253-z
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发表时间：
2014-09-30
期刊：
影响因子：
4.2
通讯作者：
Vilain S
中科院分区：
文献类型：
--
作者：
Crouzet M;Le Senechal C;Brözel VS;Costaglioli P;Barthe C;Bonneu M;Garbay B;Vilain S
细菌生物膜在自然生态系统中占主导地位，由于其对抗菌治疗，特别是对抗生素具有突出的耐药性，构成了对公共健康的威胁。到目前为止，已经开发了几种培养细菌生物膜的系统，以研究它们的表型和固着细胞的生理。虽然相关，但这种系统允许分析生物膜状态的各个方面，但通常在细菌生长几个小时后。在这里，我们描述了一种简单易用的培养铜绿假单胞菌生物膜的系统，该系统基于玻璃棉纤维的介质吸附。这种方法促进了细菌与载体的接触，使在几分钟内获得大量固着细菌成为可能。利用这个生长系统，我们证明了通过直接从固定化细胞中提取的蛋白质分离来探索生物膜形成的早期阶段的可行性。此外，蛋白质合成参与了铜绿假单胞菌的附着。我们的系统提供了足够的固着生物量，从早期孵化期开始进行生化和蛋白质组分析，从而为迄今为止无法获得的早期殖民阶段的分子分析铺平了道路。本文的在线版本(doi：10.1186/s12866-0140253-z)包含补充材料，授权用户可以使用。
Bacterial biofilms are predominant in natural ecosystems and constitute a public health threat because of their outstanding resistance to antibacterial treatments and especially to antibiotics. To date, several systems have been developed to grow bacterial biofilms in order to study their phenotypes and the physiology of sessile cells. Although relevant, such systems permit analysis of various aspects of the biofilm state but often after several hours of bacterial growth. Here we describe a simple and easy-to-use system for growing P. aeruginosa biofilm based on the medium adsorption onto glass wool fibers. This approach which promotes bacterial contact onto the support, makes it possible to obtain in a few minutes a large population of sessile bacteria. Using this growth system, we demonstrated the feasibility of exploring the early stages of biofilm formation by separating by electrophoresis proteins extracted directly from immobilized cells. Moreover, the involvement of protein synthesis in P. aeruginosa attachment is demonstrated. Our system provides sufficient sessile biomass to perform biochemical and proteomic analyses from the early incubation period, thus paving the way for the molecular analysis of the early stages of colonization that were inaccessible to date. The online version of this article (doi:10.1186/s12866-014-0253-z) contains supplementary material, which is available to authorized users.
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影响因子：
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