Conductive PPY/PDLLA conduit for peripheral nerve regeneration.
Conductive PPY/PDLLA conduit for peripheral nerve regeneration.
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DOI：
10.1016/j.biomaterials.2013.10.002
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发表时间：
2014-01
期刊：
影响因子：
14
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Xu, Haixing;Holzwarth, Jeremy M.;Yan, Yuhua;Xu, Peihu;Zheng, Hua;Yin, Yixia;Li, Shipu;Ma, Peter X.
与目前治疗临界尺寸神经缺损的方法相关的显著缺点和缺乏成功已经导致对神经组织工程的兴趣增加。导电聚合物由于其电性能而显示出巨大的前景，并且在聚吡咯（PPY）的情况下，其细胞相容性也是如此。因此，本研究的目的是合成一种导电复合神经导管与聚吡咯和聚（D，L-乳酸）（PDLLA），评估其能力，以支持大鼠嗜铬细胞瘤12（PC 12）细胞在体外分化，并确定其能力，以促进神经再生在体内。使用不同量的PPY（5%、10%和15%）来合成导管，导致不同的电导率（分别为5.65、10.40和15.56 ms/cm）。当PC 12细胞接种在这些管道和刺激100 mV的2小时，有一个显着的增加，在这两个百分比的神经突轴承细胞和中位神经突长度的PPY含量的增加。更重要的是，当PPY/PDLLA神经导管用于修复大鼠坐骨神经缺损时，其表现与金标准自体移植物相似。这些有希望的结果说明了这种PPY/PDLLA导电复合管道具有神经组织工程的潜力。
The significant drawbacks and lack of success associated with current methods to treat critically sized nerve defects have led to increased interest in neural tissue engineering. Conducting polymers show great promise due to their electrical properties, and in the case of polypyrrole (PPY), its cell compatibility as well. Thus, the goal of this study is to synthesize a conducting composite nerve conduit with PPY and poly(D, L-lactic acid) (PDLLA), assess its ability to support the differentiation of rat pheochromocytoma 12 (PC12) cells in vitro, and determine its ability to promote nerve regeneration in vivo. Different amounts of PPY (5%, 10%, and 15%) are used to synthesize the conduits resulting in different conductivities (5.65, 10.40, and 15.56 ms/cm, respectively). When PC12 cells are seeded on these conduits and stimulated with 100 mV for 2 h, there is a marked increase in both the percentage of neurite-bearing cells and the median neurite length as the content of PPY increased. More importantly, when the PPY/PDLLA nerve conduit was used to repair a rat sciatic nerve defect it performed similarly to the gold standard autologous graft. These promising results illustrate the potential that this PPY/PDLLA conducting composite conduit has for neural tissue engineering.
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影响因子：
2.9
作者：
Matsumoto, K;Ohnishi, K;Shimizu, Y
通讯作者：
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影响因子：
14
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作者：
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