Olfactory receptors are displayed on dog mature sperm cells.
Olfactory receptors are displayed on dog mature sperm cells.
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DOI：
10.1083/jcb.123.6.1441
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发表时间：
1993-12
期刊：
影响因子：
--
通讯作者：
Parmentier M
中科院分区：
文献类型：
--
作者：
Vanderhaeghen P;Schurmans S;Vassart G;Parmentier M
嗅觉受体构成了一个结构相关的G蛋白偶联受体的巨大家族，预计有多达一千个成员。我们以前已经表明，属于这个家族的基因在狗和人的雄性生殖系中表达。本研究进一步研究了这一意想不到的表达位点的功能意义。我们证明，几个狗的嗅觉受体的各个亚家族的代表基因基本上在睾丸中表达，很少或没有在嗅觉粘膜中的表达。其他随机选择的家族成员显示预期的表达位点，仅限于嗅觉系统。针对犬睾丸中最丰富表达的嗅觉受体基因的推导的氨基酸序列产生抗体。纯化的血清能够检测到的基因产物（DTMT受体）在晚轮和细长的精子细胞，以及在细胞质液滴，其特征在于成熟的狗精子细胞，和成熟精子的尾部中段。Western blotting进一步证实了成熟精子细胞膜中存在40 kD的免疫反应蛋白。总之，这些结果表明，一个大的嗅觉受体基因家族的一个子集的主要表达位点是不是嗅粘膜，但睾丸。这种表达与精子成熟过程中和成熟精子上相应蛋白的存在相关。表达模式与作为可能参与哺乳动物精子成熟、迁移和/或受精控制的未鉴定化学物质的传感器的作用一致。
Olfactory receptors constitute a huge family of structurally related G protein-coupled receptors, with up to a thousand members expected. We have shown previously that genes belonging to this family were expressed in the male germ line from both dog and human. The functional significance of this unexpected site of expression was further investigated in the present study. We demonstrate that a few dog genes representative of various subfamilies of olfactory receptors are expressed essentially in testis, with little or no expression in olfactory mucosa. Other randomly selected members of the family show the expected site of expression, restricted to the olfactory system. Antibodies were generated against the deduced amino acid sequence of the most abundantly expressed olfactory receptor gene in dog testis. The purified serum was able to detect the gene product (DTMT receptor) in late round and elongated spermatids, as well as in the cytoplasmic droplet that characterizes the maturation of dog sperm cells, and on the tail midpiece of mature spermatozoa. Western blotting further confirmed the presence of a 40-kD immunoreactive protein in the membrane of mature sperm cells. Altogether , these results demonstrate that the main expression site of a subset of the large olfactory receptor gene family is not olfactory mucosa but testis. This expression correlates with the presence of the corresponding protein during sperm cell maturation, and on mature sperm cells. The pattern of expression is consistent with a role as sensor for unidentified chemicals possibly involved in the control of mammalian sperm maturation, migration, and/or fertilization.
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影响因子：
56.9
作者：
JONES, DT;REED, RR
通讯作者：
REED, RR
DOI：
10.1016/0006-291x(89)92736-8
发表时间：
1989-12-29
影响因子：
3.1
作者：
LIBERT, F;LEFORT, A;VASSART, G
通讯作者：
VASSART, G
影响因子：
7.8
作者：
Erickson, H P
通讯作者：
Erickson, H P
影响因子：
11.4
作者：
BOEKHOFF, I;TAREILUS, E;BREER, H
通讯作者：
BREER, H
DOI：
10.1016/0006-291x(92)91606-q
发表时间：
1992-02-28
影响因子：
3.1
作者：
HAUGEN, TB;ESKILD, W;HANSSON, V
通讯作者：
HANSSON, V