Embryo sac chromosome doubling in Populus alba × P. glandulosa induced by high temperature exposure to produce triploids.
Embryo sac chromosome doubling in Populus alba × P. glandulosa induced by high temperature exposure to produce triploids.
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高温诱导白杨和腺白杨胚囊染色体加倍产生三倍体
DOI：
10.1270/jsbbs.16193
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发表时间：
2017-06
期刊：
影响因子：
2.4
通讯作者：
Zhang P
中科院分区：
文献类型：
--
作者：
Li Y;Tian M;Zhang P
确定授粉后数小时和处理持续时间对三倍体产生的影响，并揭示高温暴露下胚囊染色体加倍的有效阶段。至少对三片柳絮进行取样，并使用80个胚珠来测定胚囊发育过程。对柳絮（授粉后 2-74 小时）进行处理以诱导胚囊染色体加倍。细胞学观察表明胚囊发育是一个连续且异步的过程。授粉后50小时受精。在子代幼苗中检测到167个三倍体，三倍体产生效率最高为87.0%。在所有诱导的三倍体中，诱导胚囊染色体加倍最有效的处理时期是授粉后26～50 h，获得了121个三倍体，占所有三倍体总数的72.46%。 GLM 单变量分析表明授粉后几个小时之间存在显着差异（F = 4.516，p = 0.045）。然而，治疗持续时间之间的差异（F = 0.077，p = 0.791）并不显着。各胚囊发育阶段比例与三倍体产生比例的相关分析表明，第三次有丝分裂可能是诱导2n雌配子最有效的阶段。
To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of embryo sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the embryo sac developmental process. Catkins (2–74 h after pollination) were treated to induce embryo sac chromosome doubling. Cytological observations revealed that the embryo sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing embryo sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each embryo sac’s developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.
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DOI：
10.1080/14620316.2009.11512564
发表时间：
2009-09-01
影响因子：
1.9
作者：
Sun, Qingrong;Sun, Hongyan;Bell, Richard L.
通讯作者：
Bell, Richard L.
影响因子：
1
作者：
Zhang, S;Qi, L;Han, S
通讯作者：
Han, S
通过杨属合子染色体加倍产生四倍体
影响因子：
1.8
作者：
Wang, Jun;Shi, Le;Kang, Xiangyang
通讯作者：
Kang, Xiangyang
影响因子：
4.2
作者：
Lu, Min;Zhang, Pingdong;Chen, Yong
通讯作者：
Chen, Yong
影响因子：
6.2
作者：
Chakraborti, SP;Vijayan, K;Qadri, SMH
通讯作者：
Qadri, SMH