Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells.
Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells.
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DOI：
10.1016/j.isci.2018.05.015
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发表时间：
2018-06-29
期刊：
影响因子：
5.8
通讯作者：
Anderson HL
中科院分区：
文献类型：
--
作者：
Khadria A;Fleischhauer J;Boczarow I;Wilkinson JD;Kohl MM;Anderson HL
基于二次谐波产生（SHG）的探针可用于生物结构（诸如质膜）的非线性光学成像。合成了几种具有高二次谐波系数的两亲性卟啉染料，并对其细胞靶向性进行了研究。具有阳离子头基的探针比具有两性离子或不带电的基于乙二醇的头基的中性探针更好地定位在质膜处。仅具有双阳离子作为亲水性头部基团的卟啉染料定位在HEK 293 T细胞内以产生SHG，而三阳离子染料在体外和离体中稳固地定位在细胞（包括神经元）的质膜处。具有可忽略的荧光量子产率的三阳离子染料的铜（II）络合物作为仅SHG染料起作用。游离碱三阳离子染料已被证明用于双光子荧光和基于SHG的多模态成像。这项研究表明，有效的质膜定位的两亲性染料的疏水性和亲水性之间的平衡的重要性。合成了具有不同亲水性头基的两亲性卟啉染料膜染料疏水性和亲水性之间平衡的必要性用于多峰和仅SHG成像的远红外到近红外吸收染料展示了标记活细胞细胞内细胞器的染料的SHG化学;有机合成;化学成像方法;非线性光学
Second harmonic generation (SHG)-based probes are useful for nonlinear optical imaging of biological structures, such as the plasma membrane. Several amphiphilic porphyrin-based dyes with high SHG coefficients have been synthesized with different hydrophilic head groups, and their cellular targeting has been studied. The probes with cationic head groups localize better at the plasma membrane than the neutral probes with zwitterionic or non-charged ethylene glycol-based head groups. Porphyrin dyes with only dications as hydrophilic head groups localize inside HEK293T cells to give SHG, whereas tricationic dyes localize robustly at the plasma membrane of cells, including neurons, in vitro and ex vivo. The copper(II) complex of the tricationic dye with negligible fluorescence quantum yield works as an SHG-only dye. The free-base tricationic dye has been demonstrated for two-photon fluorescence and SHG-based multimodal imaging. This study demonstrates the importance of a balance between the hydrophobicity and hydrophilicity of amphiphilic dyes for effective plasma membrane localization. Amphiphilic porphyrin dyes with different hydrophilic head groups are synthesized Necessity of balance between hydrophobicity and hydrophilicity for membrane dyes Far-red to NIR absorbing dyes for multimodal and SHG-only imaging are presented SHG from a dye labeling the intracellular organelles of live cells is shown Chemistry; Organic Synthesis; Imaging Methods in Chemistry; Nonlinear Optics
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