Trans-illumination intestine projection imaging of intestinal motility in mice.
Trans-illumination intestine projection imaging of intestinal motility in mice.
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DOI：
10.1038/s41467-021-21930-w
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发表时间：
2021-03-16
影响因子：
16.6
通讯作者：
Xia J
中科院分区：
文献类型：
--
作者：
Wang D;Zhang H;Vu T;Zhan Y;Malhotra A;Wang P;Chitgupi U;Rai A;Zhang S;Wang L;Huizinga JD;Lovell JF;Xia J
功能性肠道显像对胃肠道疾病的诊断和治疗效果评价具有重要意义。目前，在动物模型中的肠运动的临床前成像是在麻醉下用切除的肠进行侵入性或非侵入性地进行的，并且不能揭示清醒状态下的肠动力学。利用近红外光学和高吸收造影剂，我们报告的Trans-illumination肠投影（TIP）成像系统的自由移动的小鼠。在完成系统评估后，我们进行了在体研究，获得了自由活动小鼠的运动模式。我们在体内显示典型的分段运动模式，这是以前在体外研究中显示由肠道起搏细胞控制。我们还显示了麻醉对运动模式的影响，突出了研究外在神经系统在控制运动模式中的作用的可能性，这需要未麻醉的活动物。结合光场技术，我们进一步展示了在体肠的3D成像（3D-TIP）。重要的是，增加的深度信息使我们能够提取远离腹壁的肠道，并沿着沿着不同方向量化肠道运动模式。TIP系统应开辟途径的功能成像的胃肠道在清醒的动物在自然的生理状态。目前在动物模型中的肠的临床前成像不能揭示清醒状态下的肠动力学。本文报道了一种用于自由活动小鼠的透照式肠道投影成像系统，并显示了清醒动物在自然生理状态下的肠道动力学。
Functional intestinal imaging holds importance for the diagnosis and evaluation of treatment of gastrointestinal diseases. Currently, preclinical imaging of intestinal motility in animal models is performed either invasively with excised intestines or noninvasively under anesthesia, and cannot reveal intestinal dynamics in the awake condition. Capitalizing on near-infrared optics and a high-absorbing contrast agent, we report the Trans-illumination Intestine Projection (TIP) imaging system for free-moving mice. After a complete system evaluation, we performed in vivo studies, and obtained peristalsis and segmentation motor patterns of free-moving mice. We show the in vivo typical segmentation motor pattern, that was previously shown in ex vivo studies to be controlled by intestinal pacemaker cells. We also show the effects of anesthesia on motor patterns, highlighting the possibility to study the role of the extrinsic nervous system in controlling motor patterns, which requires unanesthetized live animals. Combining with light-field technologies, we further demonstrated 3D imaging of intestine in vivo (3D-TIP). Importantly, the added depth information allows us to extract intestines located away from the abdominal wall, and to quantify intestinal motor patterns along different directions. The TIP system should open up avenues for functional imaging of the GI tract in conscious animals in natural physiological states. Current preclinical imaging of intestine in animal models cannot reveal intestinal dynamics in awake condition. Here the authors report a Transillumination Intestine Projection (TIP) imaging system for free-moving mice, and showed the intestine dynamics in conscious animal in natural physiological states.
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5.5
作者：
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通讯作者：
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