Prospective assessment of midsecretory endometrial leukemia inhibitor factor expression versus ανβ3 testing in women with unexplained infertility.
Prospective assessment of midsecretory endometrial leukemia inhibitor factor expression versus ανβ3 testing in women with unexplained infertility.
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DOI：
10.1016/j.fertnstert.2014.02.027
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发表时间：
2014-06
影响因子：
6.7
通讯作者：
Lessey, Bruce A.
中科院分区：
文献类型：
--
作者：
Franasiak, Jason M.;Holoch, Kristin J.;Yuan, Lingwen;Schammel, David P.;Young, Steven L.;Lessey, Bruce A.
目的探讨子宫内膜白血病抑制因子（LIF）在不明原因不孕症（UI）患者子宫内膜容受性中的表达。前瞻性病例对照研究。大学不孕不育诊所。1年以上尿失禁妇女和健康对照妇女。子宫内膜活检。比较两组患者的妊娠时间，评估子宫内膜LIF蛋白和ανβ3整合素的表达。应用免疫组织化学（IHC）和实时逆转录-聚合酶链反应（PCR）的信使RNA技术（实时定量逆转录- PCR）对UI妇女和健康对照妇女的子宫内膜进行评估。白血病抑制因子在对照组上皮细胞中以循环方式表达，分泌期的总体表达在对照组和UI女性之间相似，而ανβ3整合素的表达降低。然而，通过实时荧光定量PCR， ανβ3整合素表达水平低的女性的LIF信使RNA丰度比正常整合素表达的女性低4.4倍。免疫组化结果显示，异相组织中ανβ3整联素表达缺失，而LIF仅在部分样品中呈阴性表达。子宫内膜LIF表达减少与不良生殖结局密切相关。子宫内膜LIF表达在分泌中期达到高峰，在一些患有尿失禁的妇女中表达减少。在评估子宫内膜容受性时，使用LIF联合ανβ3整合素作为生物标志物似乎优于单独使用整合素检测，主要是因为其在分泌阶段的表达模式更早。
To evaluate endometrial leukemia inhibitor factor (LIF) expression as a marker of endometrial receptivity in women with unexplained infertility (UI). Prospective case-control study. University-associated infertility clinics. Women with UI for more than 1 year and healthy control women. Endometrial biopsy. Time to pregnancy was compared between patients with UI who were evaluated for endometrial LIF protein as well as ανβ3 integrin expression. Endometrium was evaluated using immunohistochemistry (IHC) and messenger RNA by real time reverse transcriptase–polymerase chain reaction (PCR) (quantitative real-time reverse transcriptase–PCR) in samples from women with UI as well as healthy control women. Leukemia inhibitor factor was expressed in epithelial cells in a cyclic fashion in controls, and overall expression in the secretory phase was similar between controls and women with UI, whereas ανβ3 integrin expression was reduced. However, using quantitative real-time PCR, LIF messenger RNA abundance was 4.4-fold lower in women with low levels of ανβ3 integrin expression compared with samples with normal integrins. By immunohistochemistry, ανβ3 integrin expression was always lacking when the histology was out of phase, whereas LIF expression was only negative in a subset of those samples. Reduced endometrial LIF expression was strongly associated with poor reproductive outcomes. Endometrial LIF expression peaks in the midsecretory phase and is reduced in some women with UI. The use of LIF in combination with ανβ3 integrin as biomarkers appears to be superior to integrin testing alone when evaluating endometrial receptivity, primarily because of its earlier pattern of expression during the secretory phase.
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影响因子：
6.7
作者：
Illera, MJ;Juan, LW;Lessey, BA
通讯作者：
Lessey, BA
影响因子：
3.8
作者：
Dimitriadis, E.;Menkhorst, E.;Paiva, P.
通讯作者：
Paiva, P.
影响因子：
6.7
作者：
Aghajanova, L;Stavreus-Evers, A;Landgren, BM
通讯作者：
Landgren, BM
影响因子：
4
作者：
Aplin, John D.;Spanswick, Catherine;Vicovac, Ljiljana
通讯作者：
Vicovac, Ljiljana
影响因子：
3.4
作者：
Dimitriadis, E;Stoikos, C;Salamonsen, LA
通讯作者：
Salamonsen, LA