Immunohistochemical localization of the NH(2)-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth.
Immunohistochemical localization of the NH(2)-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth.
复制标题
小鼠牙齿中牙本质唾液蛋白的 NH2 末端和 COOH 末端片段的免疫组织化学定位
DOI：
10.1007/s00441-012-1418-4
复制
发表时间：
2012-08
影响因子：
3.6
通讯作者：
Chen, Shuo
中科院分区：
文献类型：
--
作者：
Yuan, Guohua;Yang, Guobin;Song, Guangtai;Chen, Zhi;Chen, Shuo
牙本质唾液蛋白（DSP）是牙本质中主要的非胶原蛋白。人类的突变研究以及小鼠的基因敲除和转基因实验都证实了DSP在牙本质形成中的关键作用。我们之前的研究报道了DSP在小鼠成牙细胞样细胞中被加工成片段。为了进一步了解DSP片段的功能，我们使用免疫组织化学和western blot方法对DSP的nh2末端和cooh末端区域进行抗体检测，进一步评估了DSP在小鼠成牙细胞样细胞中的表达模式。然后采用免疫组化方法研究不同年龄小鼠牙齿中DSP nh2端、cooh端片段和骨桥蛋白（OPN）的分布。在成牙细胞样细胞中，检测到多个低分子量DSP片段，表明部分DSP蛋白在成牙细胞样细胞中被加工。在小鼠第一下颌磨牙中，抗dsp - nh2抗体在预牙本质基质中免疫反应强烈，而在矿化牙本质中免疫反应弱；相比之下，抗sp - cooh抗体在矿化牙本质，特别是牙本质小管中有较强的免疫反应，而在前牙本质中较弱。因此，成牙细胞的DSP nh2端和cooh端片段会分泌到牙齿的不同部位，提示它们可能在牙本质形成中起着不同的作用。同时，两种DSP抗体在反应性牙本质（RD）中均呈弱染色，而骨桥蛋白（OPN）在反应性牙本质（RD）中明显呈阳性。因此，DSP对RD形成的重要性可能不如OPN。
Dentin sialoprotein (DSP) is a major non-collagenous protein in dentin. Mutation studies in human, along with gene knockout and transgenic experiments in mice, have confirmed the critical role of DSP for dentin formation. Our previous study reported that DSP is processed into fragments in mouse odontoblast-like cells. In order to gain insights into the function of DSP fragments, we further evaluated the expression pattern of DSP in the mouse odontoblast-like cells using immunohistochemistry and western blot assay with antibodies against the NH2-terminal and COOH-terminal regions of DSP. Then, the distribution profiles of the DSP NH2-terminal and COOH-terminal fragments and osteopontin (OPN) were investigated in mouse teeth at different ages by immunohistochemistry. In the odontoblast-like cells, multiple low molecular weight DSP fragments were detected, suggesting that part of the DSP protein was processed in the odontoblast-like cells. In mouse first lower molars, immunoreactions for anti-DSP-NH2 antibody were intense in the predentin matrix but weak in mineralized dentin; in contrast, for anti-DSP-COOH antibody, strong immunoreactions were found in mineralized dentin, in particular dentinal tubules but weak in predentin. Therefore, DSP NH2-terminal and COOH-terminal fragments from odontoblasts were secreted to different parts of teeth, suggesting that they may play distinct roles in dentinogenesis. Meanwhile, both DSP antibodies showed weak staining in reactionary dentin (RD), whereas osteopontin (OPN) was clearly positive in RD. Therefore, DSP may be less crucial for RD formation than OPN.
登录
查看更多内容
影响因子：
1.9
作者：
Baba, O;Qin, CL;Butler, WT
通讯作者：
Butler, WT
影响因子：
1.9
作者：
Bègue-Kirn, C;Ruch, JV;Butler, WT
通讯作者：
Butler, WT
影响因子：
1.9
作者：
Butler, WT
通讯作者：
Butler, WT
DOI：
10.1016/s0934-8832(11)80030-2
发表时间：
1992-11-01
期刊：
MATRIX
影响因子：
--
作者：
BUTLER, WT;BHOWN, M;VANDIJK, S
通讯作者：
VANDIJK, S
影响因子：
2.9
作者：
Fisher, LW;Fedarko, NS
通讯作者：
Fedarko, NS