A novel sialylhexasaccharide from human milk: purification by affinity chromatography on immobilized wheat germ agglutinin.
A novel sialylhexasaccharide from human milk: purification by affinity chromatography on immobilized wheat germ agglutinin.
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人乳中新型唾液酸六糖：通过固定化麦芽凝集素亲和层析纯化。
DOI：
10.1016/0003-9861(88)90041-0
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发表时间：
1988
影响因子：
3.9
通讯作者：
Smith,DF
中科院分区：
文献类型：
--
作者：
Tarrago,MT;Tucker,KH;VanHalbeek,H;Smith,DF
如A. Kobata和V. Ginsburg [（1972）Arch.Biochem.Bioprotein.150，273-281]，并用NaB[3 H]4还原标记。当进行亲和层析固定化麦胚凝集素（WGA），一个单一的组件代表60%的S-5馏分被推迟的列。WGA延迟寡糖的脱唾液酸衍生物对WGA柱的亲和力高于天然唾液酸寡糖。中性六糖被确定为乳糖-N-新六糖的顺序外切糖苷酶diglase结合凝胶过滤分析的消化产物。通过刀豆β-半乳糖苷酶和β-N-乙酰氨基己糖苷酶酶促去除完整唾液酸低聚糖的非唾液酸化分支，得到单一唾液酸[3 H]四糖，通过与真实标准品和特异性抗体结合的共色谱法鉴定为唾液酸四糖（NeuAcα2- 6 Gal β1 -4GlcNAcβ1-3Galβ1-4GlcO[3 H]）。通过神经氨酸酶消化前后WGA纯化的寡糖的500-MHz 1H NMR光谱，获得了唾液酸六糖结构的独立证据。结构数据与人乳中以下先前未描述的唾液酸六糖一致：
A sialylhexasaccharide fraction (S-5) of human milk was obtained as described by A. Kobata and V. Ginsburg [(1972)Arch. Biochem. Biophys.150, 273–281] and labeled by reduction with NaB[3H]4. When subjected to affinity chromatography on immobilized wheat germ agglutinin (WGA), a single component representing 60% of the S-5 fraction was retarded by the column. The asialo derivative of the WGA-retarded oligosaccharide had a higher affinity for the WGA column than the native sialyloligosaccharide. The neutral hexaose was identified as lacto-N-neohexaose by sequential exoglycosidase digestions in combination with gel filtration analyses of digestion products. Enzymatic removal of the nonsialylated branch of the intact sialyloligosaccharide by jack bean β-galactosidase and β-N-acetylhexosaminidase resulted in a single sialyl[3H]tetraose which was identified as sialyltetrasaccharidec(NeuAcα2–6Galβ1–4GlcNAcβ1–3Galβ1–4GlcO[3H]) by cochromatography with authentic standard and specific antibody binding. Independent evidence for the structure of the sialylhexasaccharide was obtained by 500-MHz1H NMR spectroscopy of the WGA-purified oligosaccharide before and after neuraminidase digestion. The structural data are consistent with the following, previously undescribed, sialylhexaose in human milk:
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DOI：
10.1016/s0021-9258(19)45389-1
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
通讯作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
用抗人乳唾液酸四糖a的抗血清检测到人胎粪中的新神经节苷脂。
影响因子：
3.9
作者：
Prieto,PA;Smith,DF
通讯作者：
Smith,DF
DOI：
--
发表时间：
1979-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Baenziger;D. Fiete
通讯作者：
J. Baenziger;D. Fiete
影响因子：
2.9
作者：
SMITH, DF;ZOPF, DA;GINSBURG, V
通讯作者：
GINSBURG, V
人 SW1116 结直肠癌细胞中的 III6NeuAcLc4Cer：一种可能的癌胚抗原，不依赖于 Lewis 基因表达。
影响因子：
3.9
作者：
Law,KL;Smith,DF
通讯作者：
Smith,DF