Towards detection of biomarkers in the eye using an aptamer-based graphene affinity nanobiosensor.
Towards detection of biomarkers in the eye using an aptamer-based graphene affinity nanobiosensor.
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DOI：
10.1016/j.talanta.2022.123697
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发表时间：
2022-12-01
期刊：
影响因子：
6.1
通讯作者：
Lin, Qiao
中科院分区：
文献类型：
--
作者：
Wang, Ziran;Dai, Wenting;Yu, Shifeng;Hao, Zhuang;Pei, Renjun;De Moraes, Carlos Gustavo;Suh, Leejee H.;Zhao, Xuezeng;Lin, Qiao
我们提出了一种方法，可以使用基于适配体的石墨烯亲和纳米传感器，对眼睛中未稀释的泪液中的生物标志物进行敏感和特异性检测。该纳米传感器是一种石墨烯场效应晶体管，其中核酸适体和生物分子渗透聚乙二醇（PEG）纳米层固定在石墨烯表面。适体能够特异性识别目标生物标志物，并诱导石墨烯载体浓度的变化，通过测量其浓度来确定生物标志物的浓度。聚乙二醇纳米层最大限度地减少了样品中背景分子的非特异性吸附，否则会干扰生物标志物的检测。实验结果表明，肿瘤坏死因子α (tumor necrosis factor α, tnf - α)是一种炎症细胞因子，可在未稀释的人工泪液中进行敏感特异性检测，检测限为0.34 pM。这种检测和测量未稀释生理液体中生物标志物的能力使纳米传感器有可能用于样品稀释不实际的应用，例如可穿戴式测量眼睛中泪珠携带的生物标志物。
We present an approach to enable the sensitive and specific detection of biomarkers in undiluted tears in the eye using an aptamer-based graphene affinity nanosensor. The nanosensor is a graphene field-effect transistor, in which a nucleic acid aptamer and a biomolecule-permeable polyethylene glycol (PEG) nanolayer are immobilized on the graphene surface. The aptamer is capable of specifically recognize the target biomarker and induce a change in the carrier concentration of the graphene, which is measured to determine the biomarker concentration. The PEG nanolayer minimizes nonspecific adsorption of background molecules in the sample that would otherwise interfere with the biomarker detection. Experimental results show that tumor necrosis factor alpha (TNF-alpha), an inflammatory cytokine, can be sensitively and specifically detected in undiluted artificial tears with a limit of detection of 0.34 pM. This ability to detect and measure biomarkers in undiluted physiological fluids allows the nanosensor to be potentially used in applications where sample dilutions are not practical, such as wearable measurements of tear-borne biomarkers in the eye.
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