Acetylcholinesterase (Ace-1) target site mutation 119S is strongly diagnostic of carbamate and organophosphate resistance in Anopheles gambiae s.s. and Anopheles coluzzii across southern Ghana.
Acetylcholinesterase (Ace-1) target site mutation 119S is strongly diagnostic of carbamate and organophosphate resistance in Anopheles gambiae s.s. and Anopheles coluzzii across southern Ghana.
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乙酰胆碱酯酶（ACE-1）靶位点突变119s在Anopheles Gambiae S.S.中强烈诊断出氨基甲酸酯和有机磷酸盐的耐药性。以及加纳南部的肛门coluzzii。
DOI：
10.1186/1475-2875-12-404
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发表时间：
2013-11-09
期刊：
影响因子：
3
通讯作者：
Weetman D
中科院分区：
文献类型：
--
作者：
Essandoh J;Yawson AE;Weetman D
由于西非大部分地区对 DDT 具有高度抗药性，氨基甲酸酯和有机磷酸盐成为室内滞留喷洒 (IRS) 中拟除虫菊酯日益重要的替代品。尽管不太普遍，但在西非的冈比亚按蚊物种对（以前的 M 和 S 分子形式）中也记录了对这两种替代杀虫剂类别的抗药性。为了管理杀虫剂的功效，预测抗药性可能发生和传播的方式和地点非常重要，这可以通过使用具有高预测价值的分子诊断来帮助。按蚊和按蚊。冈比亚船队从加纳南部的 18 个地点收集并用苯灭威（最常用的氨基甲酸盐）和有机磷酸酯杀螟松进行生物测定。通过 qPCR 对 Ace-1 靶位点替换 G119S 进行基因分型。杀螟硫磷导致的死亡率高于苯虫威，尽管表型在不同人群中具有很强的相关性。 Ace-1 119S 在 An 中的出现频率要高得多。冈比亚 s.s 比 An. coluzzii，在大阿克拉的人口中超过 90%，是迄今为止报告的最高频率。 Ace-1 G119S 与两种杀虫剂的抗性密切相关，为诊断提供了很高的预测能力，尽管有一些证据表明苯虫威分子形式之间存在差异效应。该基因的测序显示，抗性等位基因缺乏变异，无法确定起源，但在加纳首次检测到 Ace-1 拷贝数变异。结果验证了 G119S 作为群体内部和群体之间有机磷和氨基甲酸酯抗性的有用诊断，同时强调了 Ace-1 基因型聚合性质的潜力，该基因型可能包含单拷贝和重复基因。现在需要进一步的工作来确定 Ace-1 重复的分布和抗性关联。
With high DDT resistance present throughout much of West Africa, carbamates and organophosphates are increasingly important alternatives to pyrethroids for indoor residual spraying (IRS). Though less widespread, resistance to both of these alternative insecticide classes has also been documented within the Anopheles gambiae species pair (formerly the M and S molecular forms) in West Africa. To manage insecticide efficacy, it is important to predict how and where resistance is likely to occur and spread, which could be aided by using molecular diagnostics with high predictive value. Anopheles coluzzii and An. gambiae s.s. were collected from 18 sites throughout southern Ghana and bioassayed with bendiocarb, the most commonly applied carbamate, and an organophosphate, fenitrothion. The Ace-1 target site substitution G119S was genotyped by qPCR. Fenitrothion induced higher mortality than bendiocarb, though phenotypes correlated strongly across populations. Ace-1 119S was found at much higher frequency in An. gambiae s.s than An. coluzzii, exceeding 90% in a population from Greater Accra, the highest frequency reported to date. Ace-1 G119S was very strongly associated with resistance to both insecticides, providing high predictive power for diagnosis, though with some evidence for a differential effect between molecular forms for bendiocarb. Sequencing of the gene revealed a lack of variation in resistant alleles precluding determination of origin, but Ace-1 copy number variation was detected for the first time in Ghana. The results validate G119S as a useful diagnostic of organophosphate and carbamate resistance within and among populations, whilst highlighting the potential for an aggregate nature of Ace-1 genotypes, which may comprise both single-copy and duplicated genes. Further work is now required to determine the distribution and resistance-association of Ace-1 duplication.
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影响因子：
3.2
作者：
Hunt RH;Fuseini G;Knowles S;Stiles-Ocran J;Verster R;Kaiser ML;Choi KS;Koekemoer LL;Coetzee M
通讯作者：
Coetzee M
DOI：
10.1016/j.cbpb.2008.03.008
发表时间：
2008-07-01
影响因子：
2.2
作者：
Alout, Haoues;Djogbenou, Luc;Weill, Mylene
通讯作者：
Weill, Mylene
影响因子：
3
作者：
Akogbeto, Martin C.;Padonou, Gil Germain;Yadouleton, Anges
通讯作者：
Yadouleton, Anges
影响因子：
1.7
作者：
Adasi, Kofi;Hemingway, Janet
通讯作者：
Hemingway, Janet
影响因子：
3.7
作者：
Kamdem C;Tene Fossog B;Simard F;Etouna J;Ndo C;Kengne P;Boussès P;Etoa FX;Awono-Ambene P;Fontenille D;Antonio-Nkondjio C;Besansky NJ;Costantini C
通讯作者：
Costantini C