Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy.
Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy.
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DOI：
10.3109/03008207.2015.1013192
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发表时间：
2015-04
影响因子：
2.9
通讯作者：
Campagnola PJ
中科院分区：
文献类型：
--
作者：
Chaudhary R;Campbell KR;Tilbury KB;Vanderby R Jr;Block WF;Kijowski R;Campagnola PJ
整个髌骨的胶原蛋白结构尚未通过3D成像进行彻底研究，其中大部分现有数据来自组织学横截面。重要的是要有一个更好的理解在正常组织中的架构，这可以应用于成像的疾病状态。为了解决这一缺点，我们研究了结合使用胶原蛋白特异性二次谐波发生（SHG）成像和测量体光学特性来表征牛关节软骨组织学定义区域的胶原纤维取向。收集来自浅、中和深区域的截面的正向和反向SHG强度作为深度的函数，并通过Monte Carlo模拟进行分析，以提取SHG产生方向，其与纤维组装有关。我们的研究结果揭示了三个区域之间的SHG正向-反向响应的差异，这些差异与先前开发的SHG发射模型一致。一些结果与其他模式的结果一致;然而，SHG分析显示中间区具有最有组织的原纤维组装。虽然不明显，但我们也报告了髌骨内这些不同区域的整体光学特性值。总的来说，这些结果提供了在不同软骨区的纤维和原纤维组件的结构变化的定量测量，并揭示了其他显微镜模式不可能的结构信息。这可以为正常软骨中的胶原纤维网络提供定量见解，最终可能被开发为骨关节炎的生物标志物。
The collagen structure throughout the patella has not been thoroughly investigated by 3D imaging, where the majority of the exiting data comes from histological cross sections. It is important to have a better understanding of the architecture in normal tissues, where this could then be applied to imaging of diseased states. To address this shortcoming, we investigated the combined use of collagen specific Second Harmonic Generation (SHG) imaging and measurement of bulk optical properties to characterize collagen fiber orientations of the histologically defined zones of bovine articular cartilage. Forward and backward SHG intensities from sections from superficial, middle and deep zones were collected as a function of depth and analyzed by Monte Carlo simulations to extract the SHG creation direction, which is related to the fibrillar assembly. Our results revealed differences in SHG forward-backward response between the three zones, where these are consistent with a previously developed model of SHG emission. Some of the findings are consistent with that from other modalities; however, SHG analysis showed the middle zone had the most organized fibril assembly. While not distinct, we also report bulk optical property values for these different zones within the patella. Collectively, these results provide quantitative measurements of structural changes at both the fiber and fibril assembly of the different cartilage zones and reveals structural information not possible by other microscope modalities. This can provide quantitative insight to the collagen fiber network in normal cartilage, which may ultimately be developed as a biomarker for osteoarthritis.
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影响因子：
3.4
作者：
Hall, Gunnsteinn;Jacques, Steven L.;Campagnola, Paul J.
通讯作者：
Campagnola, Paul J.
影响因子：
10.6
作者：
Lilledahl, Magnus B.;Pierce, David M.;Davies, Catharina de Lange
通讯作者：
Davies, Catharina de Lange
影响因子：
3.6
作者：
Hall G;Tilbury KB;Campbell KR;Eliceiri KW;Campagnola PJ
通讯作者：
Campagnola PJ
影响因子：
3.3
作者：
Sophia Fox AJ;Bedi A;Rodeo SA
通讯作者：
Rodeo SA
影响因子：
3.8
作者：
Madry, Henning;Luyten, Frank P.;Facchini, Andrea
通讯作者：
Facchini, Andrea