Identification and Mapping of the Clubroot Resistance Gene CRd in Chinese Cabbage (Brassica rapa ssp. pekinensis).
Identification and Mapping of the Clubroot Resistance Gene CRd in Chinese Cabbage (Brassica rapa ssp. pekinensis).
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大白菜根肿病抗性基因CRd的鉴定与定位
DOI：
10.3389/fpls.2018.00653
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发表时间：
2018
影响因子：
5.6
通讯作者：
Piao Z
中科院分区：
文献类型：
--
作者：
Pang W;Fu P;Li X;Zhan Z;Yu S;Piao Z
由疟原虫植物的迅速传播威胁着全球培养基的生产。链接但是，标记BRSTS61；“ 85-74”对局部病原体的“ Lab-19”，“ LNND-2”和“ Lab-10”的反应不同。 74”（CR）和“ BJN3-1” （Clubroot易感性）“ 85-74”线对局部病原体“ Lab-19”的抗性，该病原体被确定为种族4；根据CRR3的物理位置确认链接的标记。在新的中国卷心菜和其他胸前作物的新品种中，可以将CRD链接标记的识别用于标记辅助选择。
The rapid spread of clubroot disease, which is caused by Plasmodiophora brassicae, threatens Brassicaceae crop production worldwide. Breeding plants that have broad-spectrum disease resistance is one of the best ways to prevent clubroot. In the present study, eight Chinese cabbage germplasms were screened using published clubroot-resistant (CR) loci-/gene-linked markers. A CR gene Crr3 potential carrier “85-74” was detected which linked to marker BRSTS61; however, “85-74” shows different responses to local pathogens “LAB-19,” “LNND-2,” and “LAB-10” from “CR-73” which harbors Crr3. We used a next-generation sequencing-based bulked segregant analysis approach combined with genetic mapping to detect CR genes in an F2 segregant population generated from a cross between the Chinese cabbage inbred lines “85-74” (CR) and “BJN3-1” (clubroot susceptible). The “85-74” line showed resistance to a local pathogen “LAB-19” which was identified as race 4; a genetic analysis revealed that the resistance was conferred by a single dominant gene. The CR gene which we named CRd was mapped to a 60 kb (1 cM) region between markers yau389 and yau376 on chromosome A03. CRd is located upstream of Crr3 which was confirmed based on the physical positions of Crr3 linked markers. The identification of CRd linked markers can be applied to marker-assisted selection in the breeding of new CR cultivars of Chinese cabbage and other Brassica crops.
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影响因子：
3.7
作者：
Han Y;Lv P;Hou S;Li S;Ji G;Ma X;Du R;Liu G
通讯作者：
Liu G
影响因子：
3.7
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
通讯作者：
Matsumoto S
影响因子：
5.4
作者：
Rocherieux, J;Glory, P;Manzanares-Dauleux, MJ
通讯作者：
Manzanares-Dauleux, MJ
影响因子：
3.7
作者：
Chen, Jia-Yang;Tang, Yen-An;Wang, Yi-Ching
通讯作者：
Wang, Yi-Ching
影响因子：
3.1
作者：
Li, Xiaonan;Ramchiary, Nirala;Lim, Yong Pyo
通讯作者：
Lim, Yong Pyo