Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat
Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat
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面包小麦中与小麦黄花叶病毒抗性相关的数量性状基因座的定位和验证
DOI：
10.1007/s00122-011-1696-3
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发表时间：
2011-09
影响因子：
5.4
通讯作者：
Wang, Xiue
中科院分区：
文献类型：
--
作者：
Liu, Yangyang;Cheng, Shunhe;Chen, Peidu;Wang, Xiue
由小麦黄花叶病毒（WYMV）引起的小麦黄花叶病（WYM）是影响我国小麦生产的重要病害之一。本研究以西丰小麦（高抗）×镇9523（高感）的164个重组自交系（RIL）为材料，利用分子标记技术，建立了控制小麦WYMV抗性的数量性状位点（QTL）的关联分析。从4年的两个地点重复的田间试验中收集了RIL的WYMV抗性表型数据。利用JoinMap4.0软件构建了一张由273个非冗余位点组成的连锁图，该连锁图代表了小麦全部21条染色体。利用Windows QTL Cartographer V2.5软件检测到3个与WYMV抗性相关的QTL QYm.njau-3B.1、QYm. njau-5A.1和QYm. njau-7B.1，分别位于3BS、5AL和7 BS染色体上。有利等位基因效应均由西丰小麦贡献。其中，QYm.njau-3B.1和QYm. njau-5A.1在4个试验中均被检测到，分别解释了3.3-10.2%和25.9-53.7%，QYm.njau-7B.1在1个试验中均被检测到，分别解释了4.9和3.3%的表型变异。WYMV反应的大部分变异性由主效QTL QYm.njau-5A.1解释。利用F2代群体进一步验证了与QYm.njau-5A. 1连锁的分子标记与WYMV抗性的关系。结果表明，三个标记，即，Xwmc415.1、CINAU 152和CINAU 153与QYm.njau-5A.1的遗传距离分别为0.0、0.0和0.1cM，表明它们可用于小麦抗WYMV分子标记辅助选择育种。利用一组46份已知WYMV反应表型的小麦种质资源进一步验证了QYm.njau-5A. 1和上述3个标记的存在和效应。在34个抗WYMV的品种中，有12个品种含有QYm.njau-5A.1。
Wheat yellow mosaic (WYM) caused by wheat yellow mosaic bymovirus (WYMV) has been growing as one of the most serious diseases affecting wheat production in China. In this study, the association of quantitative trait loci (QTLs) governing WYMV resistance with molecular markers was established using 164 recombinant inbred lines (RILs) derived from ‘Xifeng Wheat’ (highly resistant) × ‘Zhen 9523’ (highly susceptible). Phenotypic data of WYMV resistance of the RILs were collected from 4-year, two-location replicated field trials. A molecular marker-based linkage map, which was comprised of 273 non-redundant loci and represented all the 21 wheat chromosomes, was constructed with the JoinMap 4.0 software. Using the Windows QTL Cartographer V2.5 software, three QTLs associated with WYMV resistance,QYm.njau-3B.1,QYm.njau-5A.1andQYm.njau-7B.1, were detected on chromosomes 3BS, 5AL, and 7BS, respectively. The favorable allele effects were all contributed by ‘Xifeng Wheat’. Among the three QTLs,QYm.njau-3B.1andQYm.njau-5A.1were detected in all the four trials and the overall mean, and could explain 3.3–10.2% and 25.9–53.7% of the phenotypic variation, respectively, whileQYm.njau-7B.1was detected in one trial and the overall mean and explained 4.9 and 3.3% of the phenotypic variation, respectively. A large portion of the variability for WYMV response was explained by a major QTL,QYm.njau-5A.1. The relationship of the molecular markers linked withQYm.njau-5A.1and the WYMV resistance was further validated using a secondary F2population. The results showed that three markers, i.e.,Xwmc415.1,CINAU152, andCINAU153, were closely linked toQYm.njau-5A.1with the genetic distances of 0.0, 0.0, and 0.1 cM, respectively, indicating they should be useful in marker-assisted selection (MAS) wheat breeding for WYMV resistance. A panel of germplasm collection consisting of 46 wheat varieties with known WYMV response phenotypes was further used to validate the presence and effects ofQYm.njau-5A.1and the above three markers. It was found thatQYm.njau-5A.1was present in 12 of the 34 WYMV-resistant varieties.
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DOI：
10.1007/bf02881780
发表时间：
1999-10
期刊：
Science in China Series C: Life Sciences
影响因子：
--
作者：
Jialin Yu;Liying Yan;N. Su;Z. Hou;Dawei Li;Chenggui Han;Lili Yang;Z. Cai;Yi Liu
通讯作者：
Jialin Yu;Liying Yan;N. Su;Z. Hou;Dawei Li;Chenggui Han;Lili Yang;Z. Cai;Yi Liu
影响因子：
3.1
作者：
M. Röder;V. Korzun;B. Gill;M. Ganal
通讯作者：
M. Röder;V. Korzun;B. Gill;M. Ganal
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Shengchu Wang;C. Basten;Z. Zeng
通讯作者：
Shengchu Wang;C. Basten;Z. Zeng
影响因子：
5.4
作者：
Devos, K. M.;Atkinson, M. D.;Gale, M. D.
通讯作者：
Gale, M. D.
影响因子：
5.4
作者：
E. Pestsova;E. Salina;A. Börner;V. Korzun;O. Maystrenko;M. Röder
通讯作者：
E. Pestsova;E. Salina;A. Börner;V. Korzun;O. Maystrenko;M. Röder