Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR).
Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR).
复制标题
DOI：
10.3390/biomedicines8120636
复制
发表时间：
2020-12-20
期刊：
影响因子：
4.7
通讯作者：
Rothamel D
中科院分区：
文献类型：
--
作者：
Steigmann L;Jung O;Kieferle W;Stojanovic S;Proehl A;Görke O;Emmert S;Najman S;Barbeck M;Rothamel D
到目前为止，在牙科领域，对于引导骨或组织再生（GBR/GTR），没有可生物吸收的替代品来替代不可吸收和体积稳定的膜。甚至镁（Mg）也被证明是一种有利的生物材料，用于稳定结构的发展。然而，已经描述了有必要防止过早降解以确保这种Mg植入物的功能性和生物相容性。已经开发了不同的涂层策略，但它们中的大多数都没有提供所需的功能。本研究分析了一种新的方法，离子注入（II）与PVD涂层的钝化新开发的镁膜GBR/GTR程序的基础上。为了证明镁膜的全面生物相容性和成功钝化，在体外和体内研究了未处理的镁（MG）和涂覆的镁（MG-Co）。因此，使用具有已显示的生物相容性的胶原膜作为对照材料。所有研究均根据EN ISO 10993法规进行。体外结果表明，未处理的和PVD涂层的膜都不是细胞相容性的。然而，两种膜类型均满足体内生物相容性要求。有趣的是，与未涂覆的膜相比，PVD涂层对气穴形成没有影响，但与纯Mg膜和胶原膜相比，其诱导了较低数量的抗炎巨噬细胞。相比之下，纯镁膜引起的免疫应答与胶原蛋白膜完全相当。总之，本研究表明，与不可吸收的体积稳定材料相比，纯镁膜是一种有前途的GBR/GTR治疗替代品。
To date, there are no bioresorbable alternatives to non-resorbable and volume-stable membranes in the field of dentistry for guided bone or tissue regeneration (GBR/GTR). Even magnesium (Mg) has been shown to constitute a favorable biomaterial for the development of stabilizing structures. However, it has been described that it is necessary to prevent premature degradation to ensure both the functionality and the biocompatibility of such Mg implants. Different coating strategies have already been developed, but most of them did not provide the desired functionality. The present study analyses a new approach based on ion implantation (II) with PVD coating for the passivation of a newly developed Mg membrane for GBR/GTR procedures. To demonstrate comprehensive biocompatibility and successful passivation of the Mg membranes, untreated Mg (MG) and coated Mg (MG-Co) were investigated in vitro and in vivo. Thereby a collagen membrane with an already shown biocompatibility was used as control material. All investigations were performed according to EN ISO 10993 regulations. The in vitro results showed that both the untreated and PVD-coated membranes were not cytocompatible. However, both membrane types fulfilled the requirements for in vivo biocompatibility. Interestingly, the PVD coating did not have an influence on the gas cavity formation compared to the uncoated membrane, but it induced lower numbers of anti-inflammatory macrophages in comparison to the pure Mg membrane and the collagen membrane. In contrast, the pure Mg membrane provoked an immune response that was fully comparable to the collagen membrane. Altogether, this study shows that pure magnesium membranes represent a promising alternative compared to the nonresorbable volume-stable materials for GBR/GTR therapy.
登录
查看更多内容
影响因子：
18.9
作者：
Barbeck M;Serra T;Booms P;Stojanovic S;Najman S;Engel E;Sader R;Kirkpatrick CJ;Navarro M;Ghanaati S
通讯作者：
Ghanaati S
影响因子：
2.3
作者：
Kapogianni, Eleni;Barbeck, Mike;Rothamel, Daniel
通讯作者：
Rothamel, Daniel
影响因子：
5.6
作者：
Barbeck, Mike;Kuehnel, Lennart;Jung, Ole
通讯作者：
Jung, Ole
二元镁合金的体外腐蚀与生物相容性
影响因子：
14
作者：
Gu, Xuenan;Zheng, Yufeng;Xi, Tingfei
通讯作者：
Xi, Tingfei
影响因子：
5.6
作者：
Flaig, Iris;Radenkovic, Milena;Barbeck, Mike
通讯作者：
Barbeck, Mike