Phytochemical Characterization of Mentha spicata L. Under Differential Dried-Conditions and Associated Nephrotoxicity Screening of Main Compound With Organ-on-a-Chip.
Phytochemical Characterization of Mentha spicata L. Under Differential Dried-Conditions and Associated Nephrotoxicity Screening of Main Compound With Organ-on-a-Chip.
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DOI：
10.3389/fphar.2018.01067
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发表时间：
2018
影响因子：
5.6
通讯作者：
Tian T
中科院分区：
文献类型：
--
作者：
Li X;Tian T
留兰香（Mentha spicata L.）通常用作植物调味草药。2它也有几种药理活性，对发烧，咳嗽，感染和炎症。当前的研究提出了一种非靶向比较代谢组学方法，利用HPLC-QTOF-MS高通量分析技术来深入了解干燥过程对所检查的留兰香物种的影响。据我们所知，这是第一次报告的新鲜和干燥的留兰香叶之间的成分差异确定通过代谢组学方法，揭示干叶是一个更好的生物活性代谢物的来源。采用肾芯片进一步评价山萘酚的肾毒性。在设计的芯片上，基于GelMA的3D培养平台模拟了肾脏的微环境和基本功能。此外，芯片的透明度允许在光学显微镜下直接观察。根据形态学，用30 μM山奈酚处理人胚肾细胞12 h未诱导明显的细胞损伤或细胞凋亡，因此提供了山奈酚无毒的概念验证。
Spearmint (Mentha spicata L.) is normally used as a vegetable flavoring herb. It also has several pharmacological activities against fever, cough, infection, and inflammation. The current study presents an untargeted comparative metabolomics approach utilizing HPLC-QTOF-MS high-throughput analytical technology to provide insights into the effect of the drying process on the examined spearmint species. To the best of our knowledge, this is the first report of compositional differences among fresh and dried spearmint leaves determined via a metabolomic approach to reveal that dried leaves are a better source of bioactive metabolites. The nephrotoxicity of kaempferol, a bioactive metabolite from spearmint, was further assessed with a kidney-on-a-chip. On the designed chip, a GelMA-based 3D culture platform mimics the microenvironment and basic functions of the kidney. In addition, the chip’s transparency allows for direct observation under an optical microscope. Treatment of human embryonic kidney cells with 30 μM of kaempferol for 12 h induced no obvious cell injury or apoptosis in the cells, on the basis of morphology, thus providing a proof-of-concept demonstration of kaempferol’s non-toxicity.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
14
作者：
Yue K;Trujillo-de Santiago G;Alvarez MM;Tamayol A;Annabi N;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
5.6
作者：
Yuan, Jun;Hao, Li-Jun;Qin, Min-Jian
通讯作者：
Qin, Min-Jian
丹参采后干燥过程中丹酚酸B的产生
DOI：
10.3390/molecules17032388
发表时间：
2012-02-27
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li XB;Wang W;Zhou GJ;Li Y;Xie XM;Zhou TS
通讯作者：
Zhou TS
影响因子：
9.7
作者：
Manosroi, J;Dhumtanom, P;Manosroi, A
通讯作者：
Manosroi, A