Quantitative Investigation of the Link between Actin Cytoskeleton Dynamics and Cellular Behavior.
Quantitative Investigation of the Link between Actin Cytoskeleton Dynamics and Cellular Behavior.
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肌动蛋白细胞骨架动力学与细胞行为之间联系的定量研究
DOI：
10.3390/mi13111885
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发表时间：
2022-11-01
期刊：
影响因子：
3.4
通讯作者：
Niu F
中科院分区：
文献类型：
--
作者：
Li Y;Zhuang X;Niu F
肌动蛋白相关蛋白调控的肌动蛋白细胞骨架重组与细胞生物学行为的改变密切相关。然而，对肌动蛋白机械性质如何与细胞生物学性质联系的理解仍然不清楚。本文报道了一种无标记的生物标志物，以表明这种相互关系，通过使用肌动蛋白细胞骨架模型和光镊（OT）操纵技术。生物物理和生物化学的方法，分别作为两个案例研究的刺激。通过比较电场作用下白血病细胞和人骨髓间充质干细胞（hMSC）成脂分化过程中的力学和生物学实验结果，我们认为β-actin可以作为表征actin细胞骨架力学性质变化过程中细胞生物学行为改变的指标。本研究为定量研究肌动蛋白细胞骨架重组反映细胞力学特性与细胞生物学行为之间的相互关系提供了有效途径。
Actin cytoskeleton reorganization, which is governed by actin-associated proteins, has a close relationship with the change of cell biological behavior. However, a perceived understanding of how actin mechanical property links to cell biological property remains unclear. This paper reports a label-free biomarker to indicate this interrelationship by using the actin cytoskeleton model and optical tweezers (OT) manipulation technology. Both biophysical and biochemical methods were employed, respectively, as stimuli for two case studies. By comparing the mechanical and biological experiment results of the leukemia cells under electrical field exposure and human mesenchymal stem cells (hMSC) under adipogenesis differentiation, we concluded that β-actin can function as an indicator in characterizing the alteration of cellular biological behavior during the change of actin cytoskeleton mechanical property. This study demonstrated an effective way to probe a quantitative understanding of how actin cytoskeleton reorganization reflects the interrelation between cell mechanical property and cell biological behavior.
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影响因子：
3.3
作者：
Uetaki, Megumi;Onishi, Nobuyuki;Oki, Yoshinao;Shimizu, Takatsune;Sugihara, Eiji;Sampetrean, Oltea;Watanabe, Takashi;Yanagi, Hisano;Suda, Kiyoshi;Fujii, Hiroya;Kano, Koichiro;Saya, Hideyuki;Nobusue, Hiroyuki
通讯作者：
Nobusue, Hiroyuki
影响因子：
--
作者：
Izdebska M;Zielińska W;Grzanka D;Gagat M
通讯作者：
Gagat M
影响因子：
16.6
作者：
Mahuzier A;Shihavuddin A;Fournier C;Lansade P;Faucourt M;Menezes N;Meunier A;Garfa-Traoré M;Carlier MF;Voituriez R;Genovesio A;Spassky N;Delgehyr N
通讯作者：
Delgehyr N
影响因子：
5.5
作者：
Ménasché G;Longé C;Bratti M;Blank U
通讯作者：
Blank U
影响因子：
3.9
作者：
Bai G;Li Y;Chu HK;Wang K;Tan Q;Xiong J;Sun D
通讯作者：
Sun D