Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids.
Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids.
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DOI：
10.1039/c7lc00327g
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发表时间：
2017-06-27
期刊：
影响因子：
6.1
通讯作者：
Mao L
中科院分区：
文献类型：
--
作者：
Zhao W;Cheng R;Lim SH;Miller JR;Zhang W;Tang W;Xie J;Mao L
本文报道了一种利用铁磁流体的生物相容性、无标记细胞分离方法，可以将细胞培养系中的多种低浓度癌细胞（~100个癌细胞/mL）与未稀释的白细胞分离，通量为1.2 mL/h，平均分离效率为82.2%。该分离基于癌细胞和白细胞的大小差异，并在定制的生物相容性铁磁流体中进行，该磁流体不仅保留了 7 种常用癌细胞系优异的短期生存能力，而且还保持了正常增殖。一种微流体装置经过专门设计和优化，通过消除耗时的片外样品制备和提取步骤并将其集成在芯片上以实现一步过程，从而将活细胞在铁磁流体中的暴露时间从几小时缩短到几秒钟。作为概念验证演示，该微流体装置中使用了体积分数为 0.26% 的铁磁流体，以 1.2 mL/h 的吞吐量将浓度约为 100 个细胞/mL 的细胞系中的尖峰癌细胞与白细胞分离。 A549肺癌、H1299肺癌、MCF-7乳腺癌、MDA-MB-231乳腺癌和PC-3前列腺癌细胞系的分离效率分别为80±3%、81±5%、82±5%、82±4%和86±6%。分离出的癌细胞纯度在25.3%至28.8%之间。此外，通过该策略分离的癌细胞显示出平均短期生存率为 94.4±1.3%，并且即使在分离过程之后，分离的细胞仍可被培养并表现出正常增殖至汇合。由于其优异的生物相容性和无标记操作，以及从白细胞中回收低浓度癌细胞的能力，该方法可能成为一种有前景的稀有细胞分离工具。开发了一种从白细胞中分离低浓度细胞系癌细胞的生物相容性且无标记的方法。
This paper reports a biocompatible and label-free cell separation method using ferrofluids that can separate a variety of low-concentration cancer cells from cell culture lines (~100 cancer cells/mL) from undiluted white blood cells, with a throughput of 1.2 mL/h and an average separation efficiency of 82.2%. The separation is based on size difference from cancer cells and white blood cells, and is conducted in a custom-made biocompatible ferrofluid that retains not only excellent short-term viabilities, but also normal proliferations of 7 commonly used cancer cell lines. A microfluidic device is designed and optimized specifically to shorten the exposure time of live cells in ferrofluids from hours to seconds, by eliminating time-consuming off-chip sample preparation and extraction steps and integrating them on-chip to achieve one-step process. As a proof-of-concept demonstration, a ferrofluid with 0.26% volume fraction was used in this microfluidic device to separate spiked cancer cells from cell lines at a concentration of ~100 cells/mL from white blood cells with a 1.2 mL/h throughput. The separation efficiencies were 80±3%, 81±5%, 82±5%, 82±4%, and 86±6% for A549 lung cancer, H1299 lung cancer, MCF-7 breast cancer, MDA-MB-231 breast cancer, and PC-3 prostate cancer cell lines, respectively. Separated cancer cells purity was between 25.3% and 28.8%. In addition, separated cancer cells from this strategy showed an average short-term viability of 94.4±1.3% and separated cells were cultured and demonstrated normal proliferation to the confluence even after the separation process. Owning to its excellent biocompatibility and label-free operation, and its ability to recover low concentration of cancer cells from white blood cells, this method could lead to a promising tool for rare cell separation. A biocompatible and label-free separation of low-concentration cancer cells of cell lines from white blood cells is developed.
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影响因子：
4.6
作者：
Knowlton SM;Sencan I;Aytar Y;Khoory J;Heeney MM;Ghiran IC;Tasoglu S
通讯作者：
Tasoglu S
影响因子：
4.6
作者：
Dhar M;Pao E;Renier C;Go DE;Che J;Montoya R;Conrad R;Matsumoto M;Heirich K;Triboulet M;Rao J;Jeffrey SS;Garon EB;Goldman J;Rao NP;Kulkarni R;Sollier-Christen E;Di Carlo D
通讯作者：
Di Carlo D
影响因子：
6.1
作者：
Huang, Song-Bin;Wu, Min-Hsien;Lee, Gwo-Bin
通讯作者：
Lee, Gwo-Bin
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
--
作者：
Luciani, Nathalie;Gazeau, Florence;Wilhelm, Claire
通讯作者：
Wilhelm, Claire