The Role of Bone Marrow-Derived Cells during Ectopic Bone Formation of Mouse Femoral Muscle in GFP Mouse Bone Marrow Transplantation Model.
The Role of Bone Marrow-Derived Cells during Ectopic Bone Formation of Mouse Femoral Muscle in GFP Mouse Bone Marrow Transplantation Model.
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DOI：
10.7150/ijms.24605
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发表时间：
2018
影响因子：
3.6
通讯作者：
Nagatsuka H
中科院分区：
文献类型：
--
作者：
Takabatake K;Tsujigiwa H;Song Y;Matsuda H;Kawai H;Fujii M;Hamada M;Nakano K;Kawakami T;Nagatsuka H
骨髓来源的细胞的多潜能能力已经被阐明，并且它们参与各种组织的修复和维护已经被报道。然而，骨髓源性细胞在骨生成中的作用仍然未知。在本研究中，骨髓来源的细胞在异位成骨的小鼠股骨肌肉追踪使用绿色荧光蛋白骨髓移植模型。将来自C57 BL/6-Tg（CAG-EGFP）小鼠的骨髓细胞移植到C57 BL/6 J野生型小鼠中。移植后，将不溶性骨基质（IBM）植入小鼠肌肉中。在术后第7、14和28天对异位骨形成进行组织学评估。然后进行免疫组织化学GFP单染色和GFP-骨钙素双染色。骨髓移植成功地用GFP阳性供体细胞替代了造血细胞。免疫组化分析显示，成骨细胞和骨细胞参与异位骨形成的GFP阴性，而破骨细胞和造血细胞参与骨形成的GFP阳性。这些结果表明，骨髓来源的细胞可能不会分化为成骨细胞。因此，骨髓来源的细胞在异位成骨中的主要作用可能不是通过分化成成骨细胞来诱导骨再生，而是通过将组织干细胞分化成骨细胞来促进骨形成的微环境形成。
Multipotential ability of bone marrow-derived cells has been clarified, and their involvement in repair and maintenance of various tissues has been reported. However, the role of bone marrow-derived cells in osteogenesis remains unknown. In the present study, bone marrow-derived cells during ectopic bone formation of mouse femoral muscle were traced using a GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) mice were transplanted into C57BL/6 J wild type mice. After transplantation, insoluble bone matrix (IBM) was implanted into mouse muscle. Ectopic bone formation was histologically assessed at postoperative days 7, 14, and 28. Immunohistochemistry for GFP single staining and GFP-osteocalcin double staining was then performed. Bone marrow transplantation successfully replaced hematopoietic cells with GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts and osteocytes involved in ectopic bone formation were GFP-negative, whereas osteoclasts and hematopoietic cells involved in bone formation were GFP-positive. These results indicate that bone marrow-derived cells might not differentiate into osteoblasts. Thus, the main role of bone marrow-derived cells in ectopic osteogenesis may not be to induce bone regeneration by differentiation into osteoblasts, but rather to contribute to microenvironment formation for bone formation by differentiating tissue stem cells into osteoblasts.
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影响因子：
2.9
作者：
Tsujigiwa, H;Nishizaki, K;Nagai, N
通讯作者：
Nagai, N
DOI：
10.1073/pnas.78.12.7599
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SAMPATH, TK;REDDI, AH
通讯作者：
REDDI, AH
影响因子：
15.3
作者：
Arai, F;Ohneda, O;Suda, T
通讯作者：
Suda, T
产生 IL-17 的 γδ T 细胞可促进骨再生。
影响因子：
16.6
作者：
Ono T;Okamoto K;Nakashima T;Nitta T;Hori S;Iwakura Y;Takayanagi H
通讯作者：
Takayanagi H
影响因子：
64.8
作者：
Terada, N;Hamazaki, T;Scott, EW
通讯作者：
Scott, EW