TMV recombinants encoding fused foreign transmembrane domains to the CP subunit caused local necrotic response on susceptible tobacco.

TMV recombinants encoding fused foreign transmembrane domains to the CP subunit caused local necrotic response on susceptible tobacco.
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编码与 CP 亚基融合的外源跨膜结构域的 TMV 重组体引起易感烟草的局部坏死反应。

DOI:
10.1016/j.virol.2005.11.013
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发表时间:
2006-05-10
期刊:
影响因子:
3.7
通讯作者:
Xu Z
Xu Z
中科院分区:
医学3区
文献类型:
--
作者:
Li Q;Li M;Jiang L;Zhang Q;Song R;Xu Z

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关于与外壳蛋白亚基融合的各种外源肽对相应TMV重组体侵染性的影响,发现一些TMV重组体在感病烟草上诱发局部坏死性病斑。根据SOSUI、TMpred和DAS三个程序的预测,存在一组融合外源肽含有跨膜结构域的TMV重组体。进一步的研究首次发现,TMV重组体融合肽中的外源跨膜结构域会导致感病烟草叶片的局部病变。此外,可以得出结论,没有一个TMV重组体系统感染感病烟草包含在外壳蛋白亚基的跨膜结构域。
With regard to the effects of various foreign peptides fused to the coat protein subunits on the infectivity of corresponding TMV recombinants, some of TMV recombinants were found to induce necrotic local lesions on the inoculated leaves of susceptible tobacco. This paper reported that there existed a group of TMV recombinants in which the fused foreign peptides contained a transmembrane domain according to the predictions by three programs of SOSUI, TMpred and DAS. Further studies showed for the first time that a foreign transmembrane domain in a fused peptide of the corresponding TMV recombinant would result in the local lesions on the susceptible tobacco leaves. In addition, it was concluded that none of the TMV recombinants that systematically infected susceptible tobacco contained a transmembrane domain in the coat protein subunits.
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