Restriction of cytosolic sucrose hydrolysis profoundly alters development, metabolism, and gene expression in Arabidopsis roots.
Restriction of cytosolic sucrose hydrolysis profoundly alters development, metabolism, and gene expression in Arabidopsis roots.
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DOI：
10.1093/jxb/eraa581
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发表时间：
2021-02-27
影响因子：
6.9
通讯作者：
Smith AM
中科院分区：
文献类型：
--
作者：
Pignocchi C;Ivakov A;Feil R;Trick M;Pike M;Wang TL;Lunn JE;Smith AM
拟南芥根系中胞质转化酶的减少改变了蔗糖与己糖的比例，从而导致代谢、生长、发育和基因表达模式的深刻改变。植物的根系依赖于从叶片中输入的蔗糖作为代谢和生长的基质。蔗糖及其衍生的己糖也是调节生长发育的信号分子，但对糖水平内源性变化信号的重要性了解甚少。我们报道，将蔗糖转化为己糖的胞质转化酶活性降低，导致拟南芥（拟南芥）幼苗根系代谢、生长和发育缺陷明显。除了糖和下游代谢物水平的改变外，cinv1 cinv2突变体的根还会降低伸长率、细胞和分生组织大小、异常的分生组织细胞分裂模式，以及数千种不同功能基因的表达改变。向突变体根系提供外源葡萄糖修复的缺陷相对较少。突变型和野生型根之间广泛的转录差异具有高蔗糖和低己糖信号的特征。我们得出结论，突变型表型反映了代谢和生长的低碳利用率，以及突变根细胞质中蔗糖和己糖水平升高引起的复杂糖信号。内源性蔗糖和己糖水平的这种相互变化可能提供有关糖状态的丰富信息，这些信息可转化为生长和发育的灵活调整。
A reduction in cytosolic invertase in Arabidopsis roots changes the sucrose to hexose ratio, resulting in profound alterations of metabolism, growth, development, and patterns of gene expression. Plant roots depend on sucrose imported from leaves as the substrate for metabolism and growth. Sucrose and hexoses derived from it are also signalling molecules that modulate growth and development, but the importance for signalling of endogenous changes in sugar levels is poorly understood. We report that reduced activity of cytosolic invertase, which converts sucrose to hexoses, leads to pronounced metabolic, growth, and developmental defects in roots of Arabidopsis (Arabidopsis thaliana) seedlings. In addition to altered sugar and downstream metabolite levels, roots of cinv1 cinv2 mutants have reduced elongation rates, cell and meristem size, abnormal meristematic cell division patterns, and altered expression of thousands of genes of diverse functions. Provision of exogenous glucose to mutant roots repairs relatively few of the defects. The extensive transcriptional differences between mutant and wild-type roots have hallmarks of both high sucrose and low hexose signalling. We conclude that the mutant phenotype reflects both low carbon availability for metabolism and growth and complex sugar signals derived from elevated sucrose and depressed hexose levels in the cytosol of mutant roots. Such reciprocal changes in endogenous sucrose and hexose levels potentially provide rich information about sugar status that translates into flexible adjustments of growth and development.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
6.9
作者：
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影响因子：
56.9
作者：
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通讯作者：
Benfey, Philip N.
影响因子：
7.4
作者：
Contento, AL;Kim, SJ;Bassham, DC
通讯作者：
Bassham, DC