Ultrabright fluorescent mesoporous silica nanoparticles for prescreening of cervical cancer.
Ultrabright fluorescent mesoporous silica nanoparticles for prescreening of cervical cancer.
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DOI：
10.1016/j.nano.2013.04.011
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发表时间：
2013-11
影响因子：
5.4
通讯作者：
Sokolov, I.
中科院分区：
文献类型：
--
作者：
Palantavida, Shajesh;Guz, Nataliia V.;Woodworth, C. D.;Sokolov, I.
关键词：
我们报道了最近引入的超亮荧光介孔二氧化硅纳米颗粒的第一个功能用途，它与叶酸功能化，以区分癌和癌前宫颈上皮细胞与正常细胞。粒子的高亮度有利于快速可靠地识别癌前细胞和癌细胞。从三名健康妇女和三名癌症患者中分离出正常细胞和癌细胞。用人乳头瘤病毒16型（HPV-16） DNA对正常细胞原代培养物进行永生化，获得了3株癌前细胞系。我们观察到正常细胞和癌/癌前细胞在短暂的15分钟孵育后的颗粒内化有很大的不同。与目前用于宫颈癌预筛查的HPV-DNA和细胞病理学测试相比，我们证明了我们的方法的特异性相似（94-95%），而其敏感性明显优于目前使用的测试（16%-82%）。
We report on the first functional use of recently introduced ultrabright fluorescent mesoporous silica nanoparticles, which are functionalized with folic acid, to distinguish cancerous and precancerous cervical epithelial cells from normal cells. The high brightness of the particles is advantageous for fast and reliable identification of both precancerous and cancerous cells. Normal and cancer cells were isolated from three healthy women and three cancer patients. Three precancerous cell lines were derived by immortalization of primary cultures of normal cells with human papillomavirus type-16 (HPV-16) DNA. We observed substantially different particle internalization by normal and cancerous/precancerous cells after a short incubation time of 15 minutes. Compared to HPV-DNA and cell pathology tests, which are currently used for prescreening of cervical cancer, we demonstrated that the specificity of our method was similar (94-95%), whereas its sensitivity was significantly better (95-97%) than the sensitivity of those currently used tests (16%-82%).
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影响因子：
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