Genetic elimination of field-cage populations of Mediterranean fruit flies.
Genetic elimination of field-cage populations of Mediterranean fruit flies.
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DOI：
10.1098/rspb.2014.1372
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发表时间：
2014-10-07
期刊：
影响因子：
--
通讯作者：
Alphey L
中科院分区：
文献类型：
--
作者：
Leftwich PT;Koukidou M;Rempoulakis P;Gong HF;Zacharopoulou A;Fu G;Chapman T;Economopoulos A;Vontas J;Alphey L
地中海实蝇（Medfly，Ceratitis capitata Wiedemann）是300多种水果、蔬菜和坚果的害虫。昆虫不育技术（英语：Sterile insect technique）是一种通过大量释放不育的雄性昆虫与野生雌性交配来降低种群繁殖潜力的控制措施。然而，由于大量饲养和用于灭菌的辐照过程的影响，SIT蝇可以显示出较差的现场性能。发展雌性致死的RIDL（释放携带显性致死的昆虫）菌株可以克服许多与辐射有关的SIT问题。在这里，我们提出了两个地中海果蝇RIDL菌株，OX3864A和OX3647Q的生活史特征。我们的研究结果表明：（i）RIDL的全部功能，（ii）RIDL和野生型菌株的生活史特征的等效性，以及（iii）对野生型和野生衍生的男性的高水平的性竞争力。我们还提出了第一个使用RIDL消除地中海果蝇种群的原理验证实验。每周将0X3864A雄性释放到野生型地中海实蝇的稳定种群中引起数量的连续下降，导致根除。结果表明，遗传控制可以为害虫的控制提供一种有效的替代SIT的方法。
The Mediterranean fruit fly (medfly, Ceratitis capitata Wiedemann) is a pest of over 300 fruits, vegetables and nuts. The sterile insect technique (SIT) is a control measure used to reduce the reproductive potential of populations through the mass release of sterilized male insects that mate with wild females. However, SIT flies can display poor field performance, due to the effects of mass-rearing and of the irradiation process used for sterilization. The development of female-lethal RIDL (release of insects carrying a dominant lethal) strains for medfly can overcome many of the problems of SIT associated with irradiation. Here, we present life-history characterizations for two medfly RIDL strains, OX3864A and OX3647Q. Our results show (i) full functionality of RIDL, (ii) equivalency of RIDL and wild-type strains for life-history characteristics, and (iii) a high level of sexual competitiveness against both wild-type and wild-derived males. We also present the first proof-of-principle experiment on the use of RIDL to eliminate medfly populations. Weekly releases of OX3864A males into stable populations of wild-type medfly caused a successive decline in numbers, leading to eradication. The results show that genetic control can provide an effective alternative to SIT for the control of pest insects.
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DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Gong, P;Epton, MJ;Alphey, L
通讯作者：
Alphey, L
影响因子：
46.9
作者：
Fu, Guoliang;Condon, Kirsty C.;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
1.5
作者：
Caceres, C
通讯作者：
Caceres, C
影响因子：
3.1
作者：
FRANZ, G;GENCHEVA, E;KERREMANS, P
通讯作者：
KERREMANS, P