Gastrointestinal Dynamics of Non-Encapsulated and Microencapsulated Salmonella Bacteriophages in Broiler Production.
Gastrointestinal Dynamics of Non-Encapsulated and Microencapsulated Salmonella Bacteriophages in Broiler Production.
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DOI：
10.3390/ani12020144
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发表时间：
2022-01-08
期刊：
影响因子：
--
通讯作者：
Sevilla-Navarro S
中科院分区：
文献类型：
--
作者：
Lorenzo-Rebenaque L;Malik DJ;Catalá-Gregori P;Marin C;Sevilla-Navarro S
噬菌体是杀死目标细菌的病毒，可用于治疗动物生产中的多重耐药细菌。整个肉鸡生产周期的胃肠道条件可能会影响噬菌体口服给药对沙门氏菌的有效性。微囊化的噬菌体可以保护和防止噬菌体的过早释放，从而允许靶向递送到沙门氏菌的定殖位点，盲肠。该研究设计为评估在42天生产周期中噬菌体干预的最佳时机，并比较微胶囊化的（在动物饲料中递送）和非胶囊化的（通过饮用水递送）沿胃肠道沿着递送。该研究的结果表明，Eudragit® L100 pH响应性制剂中的噬菌体的微囊化允许噬菌体靶向递送至鸡盲肠。通过动物饲料口服给药的微胶囊化沙门氏菌可能是一种有前途的方法，在动物饲养期间的任何时间在现场控制沙门氏菌。噬菌体治疗被认为是控制家禽沙门氏菌的一种有前途的工具。然而，整个生产周期中胃肠道环境条件的变化可能会影响口服给药的有效性。本研究的主要目的是评估在42天生产周期中噬菌体给药的最佳时机，并比较微囊化和非囊化的噬菌体以及噬菌体沿胃肠道沿着递送的空间和时间动力学。使用喷雾干燥方法将噬菌体FGS 011包封在pH响应性聚合物Eudragit® L100中。在不同的饲养周，15只肉仔鸡被分为三组。在24小时内，第1组接受非胶囊化的维生素B1（通过饮用水递送），第2组接受微胶囊化的维生素B1（掺入动物饲料中），第3组不接受任何维生素B1。微囊化显示能够在整个动物饲养期间将噬菌体有效递送至动物肠道和盲肠。在施用的六周期间，作物显示出两种噬菌体递送方法的最高噬菌体浓度。基于L100的包封提供了显著的保护，以使噬菌体免受PV-砂囊中的恶劣环境条件的影响（在饮用水中施用的噬菌体未观察到），这可能有助于将高噬菌体剂量递送至盲肠。未来的沙门氏菌挑战研究是必要的，以证明在饲养期间使用L100制剂微胶囊化的沙门氏菌对噬菌体治疗的益处。
Bacteriophages are viruses that kill targeted bacteria and could be used as a therapy against multidrug-resistant bacteria in animal production. Gastrointestinal tract conditions throughout the broiler production cycle might compromise the efficacy of bacteriophage oral administration against Salmonella. Microencapsulation of phages could protect and prevent the premature release of the bacteriophage, thereby allowing targeted delivery to the colonization site of Salmonella, the caecum. This study was designed to assess the optimal timing of the phage intervention over a 42-day production cycle and to compare microencapsulated (delivered in animal feed) and non-encapsulated phages (delivered through the drinking water) delivery along the gastrointestinal tract. Results of this study suggest that microencapsulation of the phages in a Eudragit® L100 pH-responsive formulation allowed targeted delivery of the phage to the chicken caecum. Microencapsulation of phages administered orally through animal feed could be a promising method to control Salmonella in the field at any time during the animal rearing period. Bacteriophage therapy is being considered as a promising tool to control Salmonella in poultry. Nevertheless, changes in gastrointestinal tract environmental conditions throughout the production cycle could compromise the efficacy of phages administered orally. The main objectives of this study were to assess the optimal timing of the phage administration over a 42-day production cycle and to compare microencapsulated and non-encapsulated phages and the spatial and temporal dynamics of the phage delivery along the gastrointestinal tract. Phage FGS011 was encapsulated in the pH-responsive polymer Eudragit® L100 using the process of spray drying. At different weeks of the chicken rearing period, 15 broilers were divided into three groups. Over a period of 24 h, group 1 received non-encapsulated phages (delivered through drinking water), group 2 received microencapsulated phages (incorporated in animal feed), and group 3 did not receive any phages. Microencapsulation was shown to enable efficient delivery of the bacteriophages to the animal gut and cecum throughout the animal rearing period. During the six weeks of application, the crop displayed the highest phage concentration for both phage delivery methods. The L100 based encapsulation offered significant protection to the phages from the harsh environmental conditions in the PV-Gizzard (not seen with phages administered in drinking water) which may help in the delivery of high phage doses to the cecum. Future Salmonella challenge studies are necessary to demonstrate the benefits of microencapsulation of phages using L100 formulation on phage therapy in field studies during the rearing period.
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影响因子：
5.7
作者：
Kempf, Florent;Menanteau, Pierrette;Velge, Philippe
通讯作者：
Velge, Philippe
影响因子：
15.8
作者：
Havelaar AH;Kirk MD;Torgerson PR;Gibb HJ;Hald T;Lake RJ;Praet N;Bellinger DC;de Silva NR;Gargouri N;Speybroeck N;Cawthorne A;Mathers C;Stein C;Angulo FJ;Devleesschauwer B;World Health Organization Foodborne Disease Burden Epidemiology Reference Group
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World Health Organization Foodborne Disease Burden Epidemiology Reference Group
影响因子：
2
作者：
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通讯作者：
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影响因子：
1.4
作者：
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通讯作者：
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影响因子：
3.2
作者：
Kim, K. H.;Ingale, S. L.;Chae, B. J.
通讯作者：
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