Feasibility of investigating the association between bacterial pathogens and oral leukoplakia in low and middle income countries: A population-based pilot study in India.
Feasibility of investigating the association between bacterial pathogens and oral leukoplakia in low and middle income countries: A population-based pilot study in India.
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DOI：
10.1371/journal.pone.0251017
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Goodman M
中科院分区：
文献类型：
--
作者：
Shridhar K;Aggarwal A;Rawal I;Gupta R;Masih S;Mehrotra R;Gillespie TW;Dhillon PK;Michaud DS;Prabhakaran D;Goodman M
某些口腔细菌病原体可能在口腔癌发生中起作用。我们评估了在印度进行一项以人群为基础的研究的可行性，以检查牙龈卟啉单胞菌，具核梭杆菌和中间普雷沃菌与口腔白斑（一种潜在的恶性疾病）和其他参与者特征的分布和水平。这项探索性病例对照研究是在一个大型的印度城市队列中进行的，数据包括22名患有口腔白斑的男性和女性（病例）和69名无白斑的对照。每名参与者提供了唾液冲洗样本，34名参与者（9例; 25名对照）的一个子集还提供了来自角化牙龈表面的牙龈拭子样本，用于定量聚合酶链反应（qPCR）。无论是病原体的分布，也不与口腔白斑的水平，但是，个别病原体水平更强烈的相互关联的情况下，与对照组相比。在对照组中，总病原体的中位数水平在社会经济地位低的人群中最高（7.55×104拷贝/ng DNA）。对于qPCR分析，唾液冲洗液提供的DNA浓度优于牙龈拭子（平均浓度：1.8 ng/μl vs. 0.2 ng/μl）。这项研究证实了在低资源环境中评估口腔微生物组的人群研究的可行性，并为未来的研究确定了有希望的线索。
Certain oral bacterial pathogens may play a role in oral carcinogenesis. We assessed the feasibility of conducting a population-based study in India to examine the distributions and levels of Porphyromonas gingivalis, Fusobacterium nucleatum and Prevotella intermedia in relation to oral leukoplakia (a potentially malignant disorder) and other participant characteristics. This exploratory case-control study was nested within a large urban Indian cohort and the data included 22 men and women with oral leukoplakia (cases) and 69 leukoplakia-free controls. Each participant provided a salivary rinse sample, and a subset of 34 participants (9 cases; 25 controls) also provided a gingival swab sample from keratinized gingival surface for quantitative polymerase chain reaction (qPCR). Neither the distribution nor the levels of pathogens were associated with oral leukoplakia; however, individual pathogen levels were more strongly correlated with each other in cases compared to controls. Among controls, the median level of total pathogens was the highest (7.55×104 copies/ng DNA) among persons of low socioeconomic status. Salivary rinse provided better DNA concentration than gingival swab for qPCR analysis (mean concentration: 1.8 ng/μl vs. 0.2 ng/μl). This study confirms the feasibility of population studies evaluating oral microbiome in low-resource settings and identifies promising leads for future research.
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