Aberrantly expressed lncRNAs and mRNAs after botulinum toxin type A inhibiting salivary secretion.
Aberrantly expressed lncRNAs and mRNAs after botulinum toxin type A inhibiting salivary secretion.
复制标题
A 型肉毒杆菌毒素抑制唾液分泌后异常表达的 lncRNA 和 mRNA。
DOI：
10.1111/odi.13633
复制
发表时间：
2020-07
期刊：
影响因子：
--
通讯作者：
Zhi-Gang Cai
中科院分区：
文献类型：
--
作者：
Qian-Ying Mao;Shang Xie;Li-Ling Wu;Ruo-Lan Xiang;Zhi-Gang Cai
目的
在这项研究中，我们试图确定长链非编码RNA（lncRNA）和信使RNA（mRNA）的表达谱，并构建功能网络来分析它们在A型肉毒杆菌毒素（BTXA）介导的唾液分泌抑制后的潜在作用。
方法
BTXA组和对照组大鼠的下颌下腺分别注射BTXA和生理盐水。采用基因芯片技术鉴定差异表达的lncRNA和mRNA。基因本体和途径分析进行了研究的生物学功能。功能网络，包括lncRNA-mRNA的共表达和竞争的内源性RNA（ceRNA）的网络，构建揭示编码和非编码基因之间的相互作用。
结果
微阵列分析显示，BTXA组和对照组之间有254个lncRNA和631个mRNA的差异表达。生物信息学分析表明，大部分的mRNA与跨膜转运蛋白的活性密切相关。构建了lncRNA-mRNA共表达和ceRNA网络，并鉴定了与唾液分泌相关的几个关键mRNA-lncRNA轴和关键microRNA。
结论
我们的研究通过微阵列分析鉴定了差异表达的lncRNA和mRNA，并通过生物信息学分析探讨了编码基因和非编码基因之间的相互作用。这些发现为BTXA介导的唾液分泌抑制机制提供了新的见解。
OBJECTIVE
In this study, we sought to determine the expression profiles of long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) and construct functional networks to analyze their potential roles following botulinum toxin type A (BTXA)-mediated inhibition of salivary secretion.
METHODS
The submandibular gland of rats in the BTXA and control groups were injected with BTXA and saline, respectively. Microarray analysis was used to identify the differentially expressed lncRNAs and mRNAs. Gene ontology and pathway analysis were performed to examine the biological functions. Functional networks, including lncRNA-mRNA co-expression and competing endogenous RNA (ceRNA) networks, were constructed to reveal the interaction between the coding and noncoding genes.
RESULTS
Microarray analysis revealed that 254 lncRNAs and 631 mRNAs were differentially expressed between the BTXA and control groups. Bioinformatic analysis revealed that most of the mRNAs were closely related to transmembrane transporter activity. lncRNA-mRNA co-expression and ceRNA networks were constructed, and several critical mRNA-lncRNA axes and key microRNAs related to salivary secretion were identified.
CONCLUSIONS
Our study identified differentially expressed lncRNAs and mRNAs through microarray analysis and explored the interactions between the coding and noncoding genes through bioinformatic analysis. These findings provide new insights into the mechanism of BTXA-mediated inhibition of salivary secretion.
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影响因子：
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