A distinct cellular profile is seen in the human endocervix during Chlamydia trachomatis infection.
A distinct cellular profile is seen in the human endocervix during Chlamydia trachomatis infection.
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DOI：
10.1111/j.1600-0897.2008.00639.x
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发表时间：
2008-11
期刊：
影响因子：
--
通讯作者：
Quayle AJ
中科院分区：
文献类型：
--
作者：
Ficarra M;Ibana JS;Poretta C;Ma L;Myers L;Taylor SN;Greene S;Smith B;Hagensee M;Martin DH;Quayle AJ
宫颈内是沙眼衣原体（CT）感染的主要目标，但对该组织的免疫库或其对这些常见细菌的反应知之甚少。使用细胞刷，我们从20名妇女在CT感染期间和抗生素治疗后的宫颈内分离细胞。同时采集宫颈拭子和血液。宫颈细胞计数，宫颈细胞和血T细胞免疫表型分析。CT通过OmpA基因序列分析分型，培养定量。CT基因型为D型、E型、F型和Ia型，感染负荷差异较大。宫颈内T细胞和中性粒细胞数量在感染期间高度升高，CD4和CD8 T细胞亚群均积累。无论有无感染，宫颈内细胞浸润均以效应记忆T细胞为主，表达CCR5和CD103的T细胞数量明显高于血液。感染期间宫颈内T细胞HLA-DR表达显著升高。人宫颈内膜对沙眼衣原体感染表现出明显的细胞反应，可通过细胞刷取样纵向评估。可以同时对感染生物体进行取样和分析。
The endocervix is a major target of Chlamydia trachomatis (CT) infection, but little is known about the immune repertoire in this tissue, or its response to these common bacteria. Using a cytobrush, we isolated cells from the endocervix of 20 women during CT infection, and post-antibiotic treatment. Endocervical swabs and blood were taken in parallel. Endocervical cells were enumerated, and endocervical and blood T cells immunophenotyped. CT was genotyped by sequence analysis of the OmpA gene, and quantified by culture. CT genotypes were D, E, F and Ia, and infectious burden varied considerably. Endocervical T cell and neutrophil numbers were highly elevated during infection, with both CD4 and CD8 T cell subsets accumulating. Regardless of the presence or absence of infection, the endocervical cell infiltrate was dominated by effector memory T cells, and the numbers of CCR5 and CD103 expressing T cells was significantly higher than in the blood. HLA-DR expression by endocervical T cells was significantly increased during infection. The human endocervix exhibits a distinct cellular response to C.trachomatis infection that can be longitudinally evaluated by cytobrush sampling. Infecting organisms can be sampled and analyzed in parallel.
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影响因子：
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