Budding yeast centromeric DNA and A+T rich bacterial DNA can function as centromeres in the fission yeast Schizosaccharomyces pombe
Budding yeast centromeric DNA and A+T rich bacterial DNA can function as centromeres in the fission yeast Schizosaccharomyces pombe
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芽殖酵母着丝粒 DNA 和富含 A T 的细菌 DNA 可以充当裂殖酵母裂殖酵母中的着丝粒
DOI：
10.1101/513150
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Barbosa A
中科院分区：
文献类型：
--
作者：
Barbosa A
真核着丝粒DNA在进化中是出了名的可变，但目前，这不能与真核着丝粒功能的保存相协调。似乎来自不同生物体的着丝粒DNA含有保守的功能重要特征，但这些特征的身份是未知的。出芽酵母（酿酒酵母）的点着丝粒和分裂酵母（酿酒酵母）的区域着丝粒经过3.5亿年的进化分离，是着丝粒DNA序列与功能之间矛盾关系的典型例子。为了在实验上解决这一矛盾，我们在裂糖菌中建立了着丝粒替代策略。着丝粒替换表明，富含A+T的细菌DNA序列具有较弱的着丝粒功能，酿酒糖酵母的着丝粒元素嵌入到非着丝粒的短序列中。pombe wee1基因的功能几乎和native一样好。pombecentromeric DNA。这些观察结果表明，着丝粒功能的决定因素在出芽酵母和裂变酵母中是共同的，并且富含A+T的DNA对于功能inS既是必要的也是充分的。非洲酒。考虑到这些酵母之间的进化距离，富含A+T的DNA很可能在多种真核生物中具有着丝粒功能。着丝粒替代使用的单向丝氨酸重组酶在许多生物体中都很有效，我们的实验策略应该允许这个想法在其他真核生物中进行测试。
Eukaryotic centromeric DNA is famously variable in evolution but currently, this cannot be reconciled with the conservation of eukaryotic centromere function. It seems likely that centromeric DNA from different organisms contains conserved functionally important features but the identity of these features is unknown. The point centromeres of the budding yeastSaccharomyces cerevisiaeand the regional centromeres of the fission yeastSchizosaccharomyces pombeare separated by 350 million years of evolution and are canonical examples of the paradoxical relationship between centromeric DNA sequence and function. We have established a centromere-replacement strategy inSchizosaccharomyces pombein order to resolve this paradox experimentally. Centromere-replacement shows that an A+T rich bacterial DNA sequence has weak centromere function and that elements of theSaccharomyces cerevisiaecentromere embedded in short sequences from the non-centromericS. pombe wee1gene function almost as well as nativeS. pombecentromeric DNA. These observations demonstrate that determinants of centromere function are held in common by the budding and fission yeasts and that A+T rich DNA is both necessary and sufficient for function inS. pombe. Given the evolutionary distance between these yeasts, it is likely that A+T rich DNA has centromere function in a wide variety of eukaryotes. Centromere-replacement uses unidirectional serine recombinases that work well in many organismsand our experimental strategy should allow this idea to be tested in other eukaryotes.
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Hegemann, JH
DOI：
10.1534/g3.111.001123
发表时间：
2011-12
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
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通讯作者：
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