Identification and classification of differentially expressed genes in pyrethroid-resistant Culex pipiens pallens
Identification and classification of differentially expressed genes in pyrethroid-resistant Culex pipiens pallens
复制标题
拟除虫菊酯抗性淡色库蚊差异表达基因的鉴定与分类
DOI：
10.1007/s00438-018-1521-7
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发表时间：
2019-03
影响因子：
3.1
通讯作者：
Zhu Chang Liang
中科院分区：
文献类型：
--
作者：
Xu Na;Sun Xiao Hong;Liu Zhi Han;Xu Yang;Sun Yan;Zhou Dan;Shen Bo;Zhu Chang Liang
淡色库蚊是传播班氏丝虫病、乙型脑炎等疾病的重要媒介，严重威胁人类健康。广泛和不适当地使用杀虫剂已造成蚊虫对杀虫剂产生抗药性，这已成为控制蚊媒疾病的重要障碍。因此，深入研究昆虫抗药性的产生机制是十分重要的。本研究的目的是根据抗性表型、基因表达谱和单核苷酸多态性（SNPs）来鉴定与杀虫剂抗性相关的基因，并筛选控制杀虫剂抗性的主效基因。利用SNP和转录组数据相结合的方法，对溴氰菊酯抗性蚊虫的基因表达数量性状位点（eQTL）进行了研究。确定了耐药组中差异最大的表达途径，并利用这些SNP和该途径中的差异表达基因（DEG）构建了调控网络。采用qPCR、siRNA显微注射和CDC瓶生物测定等方法分析了参与杀虫剂抗性控制的主要候选基因。在该途径中共鉴定出85个编码推定解毒酶的DEG（包括61个P450）。利用SNP构建了耐药调控网络，并鉴定了这些代谢基因和主要基因CYP9AL 1。通过siRNA显微注射和CDC瓶生物测定证实了CYP9AL 1在杀虫剂抗性中的功能作用。使用eQTL的方法，我们确定了重要的拟除虫菊酯抗性基因，可能有助于了解杀虫剂抗性的机制，并针对新的措施，抗性监测和管理。
Culex pipiens pallens is an important vector that transmits Bancroftian filariasis, Japanese encephalitis and other diseases that pose a serious threat to human health. Extensive and improper use of insecticides has caused insecticide resistance in mosquitoes, which has become an important obstacle to the control of mosquito-borne diseases. It is crucial to investigate the underlying mechanism of insecticide resistance. The aims of this study were to identify genes involved in insecticide resistance based on the resistance phenotype, gene expression profile and single-nucleotide polymorphisms (SNPs) and to screen for major genes controlling insecticide resistance. Using a combination of SNP and transcriptome data, gene expression quantitative trait loci (eQTLs) were studied in deltamethrin-resistant mosquitoes. The most differentially expressed pathway in the resistant group was identified, and a regulatory network was built using these SNPs and the differentially expressed genes (DEGs) in this pathway. The major candidate genes involved in the control of insecticide resistance were analyzed by qPCR, siRNA microinjection and CDC bottle bioassays. A total of 85 DEGs that encoded putative detoxification enzymes (including 61 P450s) were identified in this pathway. The resistance regulatory network was built using SNPs, and these metabolic genes, and a major gene, CYP9AL1, were identified. The functional role of CYP9AL1 in insecticide resistance was confirmed by siRNA microinjection and CDC bottle bioassays. Using the eQTL approach, we identified important genes in pyrethroid resistance that may aid in understanding the mechanism underlying insecticide resistance and in targeting new measures for resistance monitoring and management.
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影响因子：
3.7
作者：
Zhang C;Wang G;Wang J;Ji Z;Liu Z;Pi X;Chen C
通讯作者：
Chen C
影响因子：
3.7
作者：
Acevedo MA;Prosper O;Lopiano K;Ruktanonchai N;Caughlin TT;Martcheva M;Osenberg CW;Smith DL
通讯作者：
Smith DL
三个解毒基因家族的Culex pipiens quinquefasciatus的转录组和系统发育分析。
影响因子：
4.4
作者：
Yan L;Yang P;Jiang F;Cui N;Ma E;Qiao C;Cui F
通讯作者：
Cui F
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
3
作者：
Gething PW;Patil AP;Smith DL;Guerra CA;Elyazar IR;Johnston GL;Tatem AJ;Hay SI
通讯作者：
Hay SI