Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit.
Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit.
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比较代谢组学和转录组学研究揭示了有助于猕猴桃在冷胁迫下耐受冷冻的关键代谢途径
DOI：
10.3389/fpls.2021.628969
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发表时间：
2021
影响因子：
5.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Sun S;Fang J;Lin M;Hu C;Qi X;Chen J;Zhong Y;Muhammad A;Li Z;Li Y
冷胁迫对栽培猕猴桃造成严重的伤害，因为这种植物通常具有较弱的耐寒能力。然而，令人惊讶的是，猕猴桃的抗冻性的潜在机制在很大程度上仍然是未知的，特别是关于赋予这种关键耐受性的关键途径。在这里，我们研究了耐冷冻基因型KL（猕猴桃）和冷冻敏感基因型RB（A。鉴定与其抗冻性相关的主要途径和重要代谢产物。通过广泛靶向代谢组学方法，共检测到565种代谢物。在（−25°C）冷胁迫下，KEGG（京都基因和基因组百科全书）途径注释表明，KL中的类黄酮代谢途径特异性上调，这增加了其抑制活性氧（ROS）的能力。KL中确定的转录组变化伴随着可待因酮还原酶基因，查耳酮异构酶基因和花青素5-芳香酰基转移酶基因的特异性上调。RB的核苷酸代谢和酚酸代谢途径特异性上调，表明RB具有较高的能量代谢能力和较弱的休眠能力。由于LPCs（LysoPC）、LPEs（LysoPE）和游离脂肪酸在两种基因型中同时积累，因此可以作为低温冻害的生物标志物。这些关键代谢物质均参与了两种基因型猕猴桃抗寒性的调控。总之，本研究的结果证明了在冷应激条件下KL和RB之间代谢产物的组成和活性的固有差异。
Cold stress poses a serious treat to cultivated kiwifruit since this plant generally has a weak ability to tolerate freezing tolerance temperatures. Surprisingly, however, the underlying mechanism of kiwifruit’s freezing tolerance remains largely unexplored and unknown, especially regarding the key pathways involved in conferring this key tolerance trait. Here, we studied the metabolome and transcriptome profiles of the freezing-tolerant genotype KL (Actinidia arguta) and freezing-sensitive genotype RB (A. arguta), to identify the main pathways and important metabolites related to their freezing tolerance. A total of 565 metabolites were detected by a wide-targeting metabolomics method. Under (−25°C) cold stress, KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotations showed that the flavonoid metabolic pathways were specifically upregulated in KL, which increased its ability to scavenge for reactive oxygen species (ROS). The transcriptome changes identified in KL were accompanied by the specific upregulation of a codeinone reductase gene, a chalcone isomerase gene, and an anthocyanin 5-aromatic acyltransferase gene. Nucleotides metabolism and phenolic acids metabolism pathways were specifically upregulated in RB, which indicated that RB had a higher energy metabolism and weaker dormancy ability. Since the LPCs (LysoPC), LPEs (LysoPE) and free fatty acids were accumulated simultaneously in both genotypes, these could serve as biomarkers of cold-induced frost damages. These key metabolism components evidently participated in the regulation of freezing tolerance of both kiwifruit genotypes. In conclusion, the results of this study demonstrated the inherent differences in the composition and activity of metabolites between KL and RB under cold stress conditions.
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影响因子：
6.9
作者：
Krasensky J;Jonak C
通讯作者：
Jonak C
影响因子：
5.6
作者：
Ma X;Xia H;Liu Y;Wei H;Zheng X;Song C;Chen L;Liu H;Luo L
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影响因子：
11.8
作者：
Li, Hui;Ding, Yanglin;Yang, Shuhua
通讯作者：
Yang, Shuhua
影响因子：
7.3
作者：
Clemente-Moreno, Maria Jose;Omranian, Nooshin;Gago, Jorge
通讯作者：
Gago, Jorge
DOI：
10.1073/pnas.0406069101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Cook, D;Fowler, S;Thomashow, MF
通讯作者：
Thomashow, MF