Differential gene expression profiling and biological process analysis in proximal nerve segments after sciatic nerve transection.
Differential gene expression profiling and biological process analysis in proximal nerve segments after sciatic nerve transection.
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坐骨神经横断后近端神经节段的差异基因表达谱和生物过程分析
DOI：
10.1371/journal.pone.0057000
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gu X
中科院分区：
文献类型：
--
作者：
Li S;Liu Q;Wang Y;Gu Y;Liu D;Wang C;Ding G;Chen J;Liu J;Gu X
After traumatic injury, peripheral nerves can spontaneously regenerate through highly sophisticated and dynamic processes that are regulated by multiple cellular elements and molecular factors. Despite evidence of morphological changes and of expression changes of a few regulatory genes, global knowledge of gene expression changes and related biological processes during peripheral nerve injury and regeneration is still lacking. Here we aimed to profile global mRNA expression changes in proximal nerve segments of adult rats after sciatic nerve transection. According to DNA microarray analysis, the huge number of genes was differentially expressed at different time points (0.5 h–14 d) post nerve transection, exhibiting multiple distinct temporal expression patterns. The expression changes of several genes were further validated by quantitative real-time RT-PCR analysis. The gene ontology enrichment analysis was performed to decipher the biological processes involving the differentially expressed genes. Collectively, our results highlighted the dynamic change of the important biological processes and the time-dependent expression of key regulatory genes after peripheral nerve injury. Interestingly, we, for the first time, reported the presence of olfactory receptors in sciatic nerves. Hopefully, this study may provide a useful platform for deeply studying peripheral nerve injury and regeneration from a molecular-level perspective.
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DOI：
10.1083/jcb.200207050
发表时间：
2002-10-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Poliak S;Matlis S;Ullmer C;Scherer SS;Peles E
通讯作者：
Peles E
DOI：
10.1006/bbrc.1996.0580
发表时间：
1996-04-16
影响因子：
3.1
作者：
Asai, H;Kasai, H;Tsuboi, A
通讯作者：
Tsuboi, A
影响因子：
4
作者：
Goto, T;Salpekar, A;Monk, M
通讯作者：
Monk, M
影响因子：
3.3
作者：
Chen, DR;Toone, WM;Bähler, J
通讯作者：
Bähler, J
DOI：
10.1073/pnas.94.9.4766
发表时间：
1997-04-29
影响因子：
11.1
作者：
Nef, S;Nef, P
通讯作者：
Nef, P