Shedding light on host niches: label‐free in situ detection of Mycobacterium gordonae via carotenoids in macrophages by Raman microspectroscopy
Shedding light on host niches: label‐free in situ detection of Mycobacterium gordonae via carotenoids in macrophages by Raman microspectroscopy
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揭示宿主生态位：通过拉曼显微光谱通过巨噬细胞中的类胡萝卜素对戈登分枝杆菌进行无标记原位检测
作者：
A. Silge;E. Abdou;Kilian R. A. Schneider;S. Meisel;T. Bocklitz;Hui;R. Heintzmann;P. Rösch;J. Popp
大分枝杆菌是病原性分枝杆菌在感染期间的主要栖息地。目前在细胞水平上对宿主-病原体相互作用的研究仍在进行中，本研究证明了拉曼显微光谱作为一种无标记、无创的方法原位研究细胞内分枝杆菌的潜力。因此，巨噬细胞被戈登分枝杆菌感染，戈登分枝杆菌是一种已知引起与巨噬细胞细胞内存活相关的炎症的分枝杆菌。在这里，我们表明，拉曼图提供了有关细菌在二维扫描和三维图像堆栈中确定的巨噬细胞细胞边缘内的位置的空间和光谱信息。同时，细胞内的相对浓度和分布的细胞成分，如DNA，蛋白质和脂质提供了有关感染的巨噬细胞的表型信息。与细胞内一次相比，巨噬细胞外膜外或靠近外膜的细菌的位置在其光谱模式中显着不同。此外，由于细胞内微环境的化学组成，巨噬细胞内细菌的积累表现出不同的光谱/分子信息。这些数据表明，由拉曼显微光谱提供的显微镜和化学获得的信息的连接提供了一种新的分析方法来检测和表征巨噬细胞的分枝杆菌感染。
Macrophages are the primary habitat of pathogenic mycobacteria during infections. Current research about the host–pathogen interaction on the cellular level is still going on. The present study proves the potential of Raman microspectroscopy as a label‐free and non‐invasive method to investigate intracellular mycobacteria in situ. Therefore, macrophages were infected with Mycobacterium gordonae, a mycobacterium known to cause inflammation linked to intracellular survival in macrophages. Here, we show that Raman maps provided spatial and spectral information about the position of bacteria within determined cell margins of macrophages in two‐dimensional scans and in three‐dimensional image stacks. Simultaneously, the relative intracellular concentration and distributions of cellular constituents such as DNA, proteins and lipids provided phenotypic information about the infected macrophages. Locations of bacteria outside or close to the outer membrane of the macrophages were notably different in their spectral pattern compared with intracellular once. Furthermore, accumulations of bacteria inside of macrophages exhibit distinct spectral/molecular information because of the chemical composition of the intracellular microenvironment. The data show that the connection of microscopically and chemically gained information provided by Raman microspectroscopy offers a new analytical way to detect and to characterize the mycobacterial infection of macrophages.
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影响因子：
4.3
作者：
Hartmann, Katharina;Becker-Putsche, Melanie;Bocklitz, Thomas;Pachmann, Katharina;Niendorf, Axel;Roesch, Petra;Popp, Juergen
通讯作者：
Popp, Juergen
影响因子：
6.7
作者：
Cooke DE;Cano LM;Raffaele S;Bain RA;Cooke LR;Etherington GJ;Deahl KL;Farrer RA;Gilroy EM;Goss EM;Grünwald NJ;Hein I;MacLean D;McNicol JW;Randall E;Oliva RF;Pel MA;Shaw DS;Squires JN;Taylor MC;Vleeshouwers VG;Birch PR;Lees AK;Kamoun S
通讯作者：
Kamoun S
影响因子：
4.9
作者：
Matthaeus, Christian;Kale, Amit;Diem, Max
通讯作者：
Diem, Max
拉曼光谱检测镍对单个链霉菌细胞的影响——重金属污染的可能生物指示剂
影响因子：
2.5
作者：
Walter A;Kuhri S;Reinicke M;Bocklitz T;Schumacher W;Rösch P;Merten D;Büchel G;Kothe E;Popp J
通讯作者：
Popp J