Neurokinin A in capsaicin-sensitive neurons of the guinea-pig inferior mesenteric ganglia: an additional putative mediator for the non-cholinergic excitatory postsynaptic potential.
Neurokinin A in capsaicin-sensitive neurons of the guinea-pig inferior mesenteric ganglia: an additional putative mediator for the non-cholinergic excitatory postsynaptic potential.
复制标题
豚鼠肠系膜下神经节辣椒素敏感神经元中的神经激肽 A：非胆碱能兴奋性突触后电位的另一个假定介质。
DOI：
10.1016/0306-4522(87)90050-9
复制
发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Lundberg,JM
中科院分区：
文献类型：
--
作者：
Saria,A;Ma,RC;Dun,NJ;Theodorsson-Norheim,E;Lundberg,JM
用放射免疫法检测了豚鼠肠系膜下神经节神经激肽A样免疫反应性的存在。在实验前7天用辣椒素预处理动物，使神经激肽A样免疫反应性的平均含量从每个神经节150 ± 31.3 fmol的对照值降低了85%。高效液相色谱法显示，神经激肽-A样免疫反应是异质性的，除了神经激肽A，对应于两栖动物速激肽eledoisin和神经肽K的峰被检测到，他们也被耗尽辣椒素。电生理研究表明，神经激肽A无论是通过灌流或压力喷射应用诱发的肠系膜下神经节神经元在大多数体外缓慢的去极化。神经激肽-A-诱发的去极化在大多数细胞测试与膜输入电阻的小幅增加。然而，膜超极化增加了反应;神经激肽A诱导的去极化的外推平均平衡电位为− 36 mV。去除细胞外钠离子而不是氯离子抑制神经激肽A诱导的去极化。在神经激肽A的存在下，由外源性P物质或由腹下神经的重复刺激引起的缓慢去极化被可逆地消除。我们的研究表明，在豚鼠的辣椒素敏感纤维中，神经激肽A样免疫反应性可能与P物质样免疫反应性共存，猪椎前神经节和神经激肽A的作用一方面和P物质的作用另一方面的相似性提出了非-在肠系膜下神经节中引起的胆碱能兴奋电位可能不是由一种而是由许多密切相关的速激肽产生。
The presence of neurokinin-A-like immunoreactivity in guinea-pig inferior mesenteric ganglia was detected by radioimmunoassay procedures. Pretreating the animals with capsaicin 7 days prior to experimentations reduced the mean content of neurokinin-A-like immunoreactivity by 85% from its control value of150 ± 31.3 fmol per ganglion. High-performance liquid chromatography revealed that neurokinin-A-like immunoreactivity was heterogenous as in addition to neurokinin A, peaks corresponding to the amphibian tachykinin eledoisin and to neuropeptide K were detected, and they too were depleted by capsaicin. Electrophysiological studies showed that neurokinin A applied either by superfusion or by pressure ejection evoked a slow depolarization in the majority of inferior mesenteric ganglia neuronsin vitro. Neurokinin-A-evoked depolarizations in the majority of cells tested were associated with a small increase in membrane input resistance. However, the responses were increased by membrane hyperpolarization; the extrapolated mean equilibrium potential of neurokinin-A-induced depolarization was − 36 mV. Removal of extracellular sodium but not chloride ions suppressed the neurokinin-A-induced depolarization. The slow depolarization elicited either by exogenously applied substance P or by repetitive stimulation of hypogastric nerves was reversibly eliminated in the presence of neurokinin A.Collectively, our studies suggest that neurokinin-A-like immunoreactivity may coexist with substance-P-like immunoreactivity in capsaicin-sensitive fibers in the guinea-pig prevertebral ganglia and that the similarity of the actions of neurokinin A on the one hand and substance P on the other raises the possibility that non-cholinergic excitatory potentials elicited in the inferior mesenteric ganglia may be generated by not one but a number of closely related tachykinins.
登录
查看更多内容
使用与磁性微球缀合的单克隆抗体从骨髓中去除神经母细胞瘤细胞
DOI：
10.1016/s0140-6736(84)90004-7
发表时间：
1984
期刊：
The Lancet
影响因子：
--
作者：
J. Treleaven;J. Ugelstad;T. Philip;F. Gibson;A. Rembaum;G. Caine;J. Kemshead
通讯作者：
J. Kemshead
影响因子：
56.9
作者：
P. Rous;J. W. Bearad
通讯作者：
J. W. Bearad
通过细胞折射法和核磁共振测量恶性组织中的水
影响因子：
2
作者：
K. Ross;R. Gordon
通讯作者：
R. Gordon
影响因子：
3.5
作者：
MOSBACH, K;SCHRODER, U
通讯作者：
SCHRODER, U
影响因子：
3.8
作者：
E. Sada;S. Katoh;M. Terashima
通讯作者：
M. Terashima