Cell cycle-specific changes in histone phosphorylation associated with cell proliferation and chromosome condensation.
Cell cycle-specific changes in histone phosphorylation associated with cell proliferation and chromosome condensation.
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DOI：
10.1083/jcb.60.2.356
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发表时间：
1974-02
期刊：
影响因子：
--
通讯作者：
Tobey RA
中科院分区：
文献类型：
--
作者：
Gurley LR;Walters RA;Tobey RA
采用制备性聚丙烯酰胺凝胶电泳法检测同步型中国仓鼠细胞（CHO系）组蛋白磷酸化水平。结果表明，组蛋白f1磷酸化在g1阻滞细胞和早期g1穿越细胞中不存在，在穿越细胞进入S期前2小时开始。因此，在S期新f1合成之前，f1磷酸化是发生在旧f1上的非增殖细胞向增殖细胞转化的最早的生化事件之一。结果还表明，f3和f1的一个亚片段仅在细胞跨越G2/M边界并穿越前期时快速磷酸化。由于这些磷酸化事件不发生在G1， S或G2，并且在中期大大减少，因此可以得出结论，这两个特定的磷酸化事件与间期染色质凝聚成有丝分裂染色体有关。在中期细胞向G1间期细胞转变过程中，这些特定组蛋白片段中预标记的32PO4缺失支持了这一结论。组蛋白磷酸化与细胞周期的关系模型提出了f1磷酸化参与染色质结构变化与连续间期“染色体周期”，最终在有丝分裂与f3和f1磷酸化介导的染色体凝聚。
Preparative polyacrylamide gel electrophoresis was used to examine histone phosphorylation in synchronized Chinese hamster cells (line CHO). Results showed that histone f1 phosphorylation, absent in G1-arrested and early G1-traversing cells, commences 2 h before entry of traversing cells into the S phase. It is concluded that f1 phosphorylation is one of the earliest biochemical events associated with conversion of nonproliferating cells to proliferating cells occurring on old f1 before synthesis of new f1 during the S phase. Results also showed that f3 and a subfraction of f1 were rapidly phosphorylated only during the time when cells were crossing the G2/M boundary and traversing prophase. Since these phosphorylation events do not occur in G1, S, or G2 and are reduced greatly in metaphase, it is concluded that these two specific phosphorylation events are involved with condensation of interphase chromatin into mitotic chromosomes. This conclusion is supported by loss of prelabeled 32PO4 from those specific histone fractions during transition of metaphase cells into interphase G1 cells. A model of the relationship of histone phosphorylation to the cell cycle is presented which suggests involvement of f1 phosphorylation in chromatin structural changes associated with a continuous interphase "chromosome cycle" which culminates at mitosis with an f3 and f1 phosphorylation-mediated chromosome condensation.
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DOI：
10.1016/0006-291x(70)91027-2
发表时间：
1970-01-01
影响因子：
3.1
作者：
GURLEY, LR;WALTERS, RA;ENGER, MD
通讯作者：
ENGER, MD
影响因子：
3.9
作者：
GURLEY, LR;WALTERS, RA;TOBEY, RA
通讯作者：
TOBEY, RA
DOI：
10.1073/pnas.69.8.2224
发表时间：
1972-01-01
影响因子：
11.1
作者：
PEDERSON, T
通讯作者：
PEDERSON, T
DOI：
10.1111/j.1432-1033.1973.tb02664.x
发表时间：
1973-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BRADBURY, EM;INGLIS, RJ;SARNER, N
通讯作者：
SARNER, N
影响因子：
2.9
作者：
ENGER, MD;TOBEY, RA
通讯作者：
TOBEY, RA