Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and Their In Vivo Multiple Biological Applications.
Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and Their In Vivo Multiple Biological Applications.
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DOI：
10.1155/2022/5994033
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发表时间：
2022
影响因子：
--
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中科院分区：
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我们提出了一种低成本、环保且高效的细菌介导合成氧化锌纳米粒子（ZnO-NP）的方法，利用苯甲酸副梭菌菌株 5610 作为封端剂和还原剂。使用扫描电子显微镜、X射线衍射、傅里叶变换红外光谱、能量色散X射线和紫外可见光谱对生物合成的ZnO-NPs进行物理化学表征。使用紫外-可见光谱观察到 441 nm 处的主要窄峰，验证了纳米颗粒的存在。根据SEM和TEM研究，ZnO-NPs的平均尺寸为50纳米。通过X射线衍射分析确定晶体尺寸为48.22nm。 FTIR 分析证实了 ZnO-NP 表面存在多种还原代谢物。研究了合成的纳米颗粒针对猪螺杆菌、布氏螺杆菌、猫螺杆菌和所罗门螺杆菌的生物活性。猪螺杆菌是最脆弱的菌株，在 5 mg/mL 剂量下的抑菌圈为 19.53 ± 0.62 mm。抗炎和大鼠爪水肿实验结果表明，细菌介导的ZnO-NPs具有很强的抑制作用。在关节炎模型中，与标准药物相比，21 天后 ZnO-NPs 溶液显示出 87.62 ± 0.12% 的水肿抑制效果。在抗糖尿病试验中，ZnO-NPs 显着降低了 STZ 诱导的糖尿病小鼠的血糖水平。在这项研究中，还测定了人类红细胞的颗粒生物相容性。考虑到ZnO-NPs的生物学重要性，我们很容易得出这样的结论：苯甲酸副梭菌菌株5610介导的ZnO-NPs将成为一种有前景的抗糖尿病、抗菌、抗关节炎和抗炎剂体内实验模型，并可用作有效的抗糖尿病药物。
We presented a low-cost, eco-friendly, and efficient bacterium-mediated synthesis of zinc oxide nanoparticles (ZnO-NPs) utilizing Paraclostridium benzoelyticum strain 5610 as a capping and reducing agent. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray, and UV-vis spectroscopy were used to physiochemically characterize the biosynthesized ZnO-NPs. A major narrow peak at 441 nm was observed using UV-visible spectroscopy, verifying the presence of nanoparticles. According to SEM and TEM studies, the average dimensions of ZnO-NPs was 50 nm. The crystal size of 48.22 nm was determined by XRD analysis. FTIR analysis confirmed the presence of various reducing metabolites on the surface of ZnO-NPs. The synthesized nanoparticles were investigated for biological activity against Helicobacter suis, Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis. Helicobacter suis was the most vulnerable strain, with an inhibitory zone of 19.53 ± 0.62 mm at 5 mg/mL dosage. The anti-inflammatory and the findings of the rat paw edema experiments revealed that the bacterium-mediated ZnO-NPs had a strong inhibitory action. In the arthritis model, the solution of ZnO-NPs showed 87.62 ± 0.12% inhibitory effect of edema after 21 days when linked with that of the standard drug. In the antidiabetic assay, ZnO-NPs sharply reduced glucose level in STZ-induced diabetic mice. In this study, the particle biocompatibility by human red blood cells was also determined. Keeping in view the biological importance of ZnO-NPs, we may readily get the conclusion that Paraclostridium benzoelyticum strain 5610-mediated ZnO-NPs will be a prospective antidiabetic, antibacterial, antiarthritic, and anti-inflammatory agent in vivo experimental models and can be used as a potent antidiabetic drug.
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影响因子：
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