Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. Morton) extract and its fractions.
Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. Morton) extract and its fractions.
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DOI：
10.1021/jf104640k
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发表时间：
2011-03-23
影响因子：
6.1
通讯作者：
Qian SY
中科院分区：
文献类型：
--
作者：
Zou Y;Chang SK;Gu Y;Qian SY
用酸化丙酮水溶液提取小扁豆中的酚类化合物。将粗Morton提取物（CME）涂于大树脂柱上，用甲醇水溶液解吸得到半纯化的Morton提取物（SPME）。SPME在Sephadex LH-20色谱柱上进一步分离为5个主要组分（Fr I - Fr V）。用比色法测定了其总酚含量（TPC）、总黄酮含量（TFC）、缩合单宁含量（CTC）等植物化学成分。通过DPPH清除活性、trolox当量抗氧化能力（TEAC）、铁还原抗氧化能力（FRAP）和氧自由基吸收能力（ORAC）等方法筛选提取物和馏分的抗氧化活性。并采用HPLC-DAD、HPLC-MS等方法对有效部位的成分进行了测定。结果表明，浓缩单宁（Fr V）组分的TPC、CTC值显著高于粗提物、SPME和低分子量组分（Fr I ~ IV），抗氧化活性显著提高。其中有18个化合物，其中17个化合物通过紫外和质谱初步鉴定。HPLC-MS分析显示Frⅱ主要含山奈酚苷，Frⅲ和Frⅳ主要含黄酮类苷，Fr V主要由缩合单宁组成。结果表明，扁豆提取物是一种很有前途的抗氧化酚类物质来源，可以作为一种促进健康的膳食补充剂。
Phenolic compounds were extracted from Morton lentils using acidified aqueous acetone. The crude Morton extract (CME) was applied onto a macroresin column and desorbed by aqueous methanol to obtain a semi-purified Morton extract (SPME). The SPME was further fractionated over Sephadex LH-20 column into five main fractions (Fr I – Fr V). The phytochemical contents such as total phenolic content (TPC), total flavonoid content (TFC), and condensed tannin content (CTC) of the CME, SPME, and its fractions were examined by colorimetric methods. Antioxidant activity of extracts and fractions were screened by DPPH scavenging activity, trolox equivalent antioxidant capacity (TEAC), ferric reduced antioxidant power (FRAP), and oxygen radical absorbing capacity (ORAC) methods. In addition, the compositions of active fractions were determined by HPLC-DAD and HPLC-MS methods. Results showed that fraction enriched in condensed tannins (Fr V) exhibited significantly higher value of TPC, CTC and higher antioxidant activity as compared to the crude extract, SPME and low-molecular-weight fractions (Fr I – IV). Eighteen compounds existed in those fractions, and seventeen were tentatively identified by UV and MS spectra. HPLC-MS analysis revealed Fr II contained mainly kaempferol glycoside, Fr III and Fr IV mainly contained flavonoid glycosides, and Fr V was composed of condensed tannins. The results suggested that extract of Morton lentils is a promising source of antioxidant phenolics, and may be used as a dietary supplement for health promotion.
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影响因子：
6.1
作者：
Tang, Xueyan;He, Zhiyong;Chen, Jie
通讯作者：
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影响因子：
4.1
作者：
Waridel, P;Wolfender, JL;Hostettmann, K
通讯作者：
Hostettmann, K
影响因子：
3.6
作者：
Franks, PW;Wong, MY;Wareham, NJ
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影响因子：
6.1
作者：
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通讯作者：
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影响因子：
6.1
作者：
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通讯作者：
Sutherland, Daniel H.