Impaired trigeminal control of ingestive behavior in the Prrxl1-/- mouse is associated with a lemniscal-biased orosensory deafferentation.
Impaired trigeminal control of ingestive behavior in the Prrxl1-/- mouse is associated with a lemniscal-biased orosensory deafferentation.
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DOI：
10.1371/journal.pone.0258837
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Zeigler HP
中科院分区：
文献类型：
--
作者：
Resulaj A;Wu J;Hartmann MJZ;Feinstein P;Zeigler HP
尽管外周传入神经阻滞研究已证明三叉神经传入在调节饮食的口感觉运动控制中发挥关键作用，但介导该控制的中枢三叉神经通路以及控制的时间尺度仍有待阐明。在啮齿类动物中，三个上行体感通路处理和传递口面部机械感觉输入：丘系、系旁系和系外。其中两条通路（丘系和系外）表现出口面部感觉表面的高度结构化的地形表征，例如动物面部触须与脑干中的桶状体、丘脑中的桶状体和皮质中的桶状体之间的一对一体位映射。在这里，我们使用 Prrxl1 敲除小鼠模型（也称为 DRG11 敲除）来研究可能与丘系通路破坏相关的摄入行为缺陷。 Prrxl1 缺失破坏了整个丘系通路的体位模式和轴突投射，但保留了丘系外核的模式。我们的数据揭示了一种不精确且低效的摄取表型。饮酒行为在毫秒到秒的时间尺度上表现出缺陷。饮食行为在更广泛的时间范围内表现出缺陷。对食物获取和消耗率的分析表明在秒的时间尺度上存在缺陷，对体重的分析表明在长期食欲控制的尺度上存在缺陷。我们认为，沿着丘系通路的三叉神经感觉信息的有序组装对于驱动饮食动作序列的运动回路的快速和精确调节至关重要。
Although peripheral deafferentation studies have demonstrated a critical role for trigeminal afference in modulating the orosensorimotor control of eating and drinking, the central trigeminal pathways mediating that control, as well as the timescale of control, remain to be elucidated. In rodents, three ascending somatosensory pathways process and relay orofacial mechanosensory input: the lemniscal, paralemniscal, and extralemniscal. Two of these pathways (the lemniscal and extralemniscal) exhibit highly structured topographic representations of the orofacial sensory surface, as exemplified by the one-to-one somatotopic mapping between vibrissae on the animals’ face and barrelettes in brainstem, barreloids in thalamus, and barrels in cortex. Here we use the Prrxl1 knockout mouse model (also known as the DRG11 knockout) to investigate ingestive behavior deficits that may be associated with disruption of the lemniscal pathway. The Prrxl1 deletion disrupts somatotopic patterning and axonal projections throughout the lemniscal pathway but spares patterning in the extralemniscal nucleus. Our data reveal an imprecise and inefficient ingestive phenotype. Drinking behavior exhibits deficits on the timescales of milliseconds to seconds. Eating behavior shows deficits over an even broader range of timescales. An analysis of food acquisition and consummatory rate showed deficits on the timescale of seconds, and analysis of body weight suggested deficits on the scale of long term appetitive control. We suggest that ordered assembly of trigeminal sensory information along the lemniscal pathway is critical for the rapid and precise modulation of motor circuits driving eating and drinking action sequences.
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影响因子：
5.3
作者：
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