Dynamic integration and excision of filamentous phage XacF1 in Xanthomonas citri pv. citri, the causative agent of citrus canker disease.
Dynamic integration and excision of filamentous phage XacF1 in Xanthomonas citri pv. citri, the causative agent of citrus canker disease.
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DOI：
10.1002/2211-5463.12312
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发表时间：
2017-11
期刊：
影响因子：
2.6
通讯作者：
Yamada T
中科院分区：
文献类型：
--
作者：
Ahmad AA;Kawabe M;Askora A;Kawasaki T;Fujie M;Yamada T
Inovirus XacF 1（7325个核苷酸）整合到柑橘黄单胞菌致病变种（Xanthomonas citri pv.）柑橘属（Citri）菌株通过宿主XerC/D重组系统在宿主的dif位点（attB）上进行了重组。XacF 1 attP序列位于ORF 12的编码区内，ORF 12是一种可能的噬菌体调节因子。整合后，该开放阅读框（ORF）在宿主基因组上被分成两部分。我们检测了Xcc菌株MAFF 301080中XacF 1的动态整合/切除，发现整合开始于感染后4 h（p.i.）并在12 h p.i.此后，整合与游离形式的比例保持恒定，表明宿主基因组中XacF 1整合和切除的平衡。然而，在XacF 1中ORF 12的5′-缺失后，整合状态变得非常不稳定，表明ORF 12在Xcc菌株中XacF 1的整合周期中起关键作用。
Inovirus XacF1 (7325 nucleotides) is integrated into the genome of Xanthomonas citri pv. citri (Xcc) strains at the host dif site (attB) by the host XerC/D recombination system. The XacF1 attP sequence is located within the coding region of ORF12, a possible phage regulator. After integration, this open reading frame (ORF) is split into two pieces on the host genome. We examined dynamic integration/excision of XacF1 in Xcc strain MAFF 301080 and found that the integration started at 4 h postinfection (p.i.) and peaked at 12 h p.i. Thereafter, the ratio of integrated to free forms remained constant, suggesting equilibrium of integration and excision of XacF1 in the host genome. However, the integrated state became very unstable following a 5′‐deletion of ORF12 in XacF1, suggesting that ORF12 plays a key role in the integration cycle of XacF1 in Xcc strains.
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