Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related to Fusarium Wilt Disease Resistance and Susceptibility in Two Vernicia Species.
Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related to Fusarium Wilt Disease Resistance and Susceptibility in Two Vernicia Species.
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DOI：
10.3389/fpls.2016.01974
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发表时间：
2016
影响因子：
5.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Chen Y;Yin H;Gao M;Zhu H;Zhang Q;Wang Y
油桐是一种很有前途的经济作物。然而，由于枯萎病的严重危害，其姊妹种油桐（Vernicia montana）对枯萎病的抵抗力却很强。然而，这种差异背后的遗传机制在很大程度上仍然未知。我们在这里产生了比较转录组图谱的不同阶段的尖孢镰刀菌感染油桐根。福特弧菌和蒙塔纳弧菌的转录组从头组装，分别含有258，430和245，240个非冗余转录本，N50值分别为1776和2452。在油桐属物种中共鉴定出44，310对推定的一对一直向同源基因。总体而言，绝大多数的orthopathy基因共享一个非常相似的表达模式。通过定量实时PCR进一步验证了一小组基因的表达模式。此外，157 unigenes的表达显着相关的两个物种之间被定义，基因集富集分析表明，在增加防御反应和茉莉酸和水杨酸的信号反应在病原体攻击的作用。共表达网络分析进一步鉴定了17个中心单基因，如丝氨酸/苏氨酸蛋白激酶D 6PK、富含亮氨酸重复序列的受体样激酶（LRR-RLK）和EREBP转录因子，它们在植物病原体抗性中发挥重要作用。有趣的是，大多数枢纽基因的表达显着差异之间的V. montana和V. fordii。基于我们的研究结果，我们提出了一个模型来描述主要的分子反应，抵抗V. montana的防御反应对F。尖孢菌属这些数据代表了培育更具病原体抗性的福特葡萄的关键一步。
Vernicia fordii (tung oil tree) is a promising industrial crop. Unfortunately, the devastating Fusarium wilt disease has caused its great losses, while its sister species (Vernicia montana) is remarkably resistant to this pathogen. However, the genetic mechanisms underlying this difference remain largely unknown. We here generated comparative transcriptomic atlases for different stages of Fusarium oxysporum infected Vernicia root. The transcriptomes of V. fordii and V. montana were assembled de novo and contained 258,430 and 245,240 non-redundant transcripts with N50 values of 1776 and 2452, respectively. A total of 44,310 pairs of putative one-to-one orthologous genes were identified in Vernicia species. Overall, the vast majority of orthologous genes shared a remarkably similar expression mode. The expression patterns of a small set of genes were further validated by quantitative real-time PCR. Moreover, 157 unigenes whose expression significantly correlated between the two species were defined, and gene set enrichment analysis indicated roles in increased defense response and in jasmonic and salicylic acid signaling responses during pathogen attack. Co-expression network analysis further identified the 17 hub unigenes, such as the serine/threonine protein kinase D6PK, leucine-rich repeat receptor-like kinase (LRR-RLK), and EREBP transcription factor, which play essential roles in plant pathogen resistance. Intriguingly, the expression of most hub genes differed significantly between V. montana and V. fordii. Based on our results, we propose a model to describe the major molecular reactions that underlie the defense responses of resistant V. montana to F. oxysporum. These data represent a crucial step toward breeding more pathogen-resistant V. fordii.
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影响因子：
4.6
作者：
Chen YC;Wong CL;Muzzi F;Vlaardingerbroek I;Kidd BN;Schenk PM
通讯作者：
Schenk PM
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
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影响因子：
11.6
作者：
Anderson, JP;Badruzsaufari, E;Kazan, K
通讯作者：
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影响因子：
9.4
作者：
Horvath, David P.;Hansen, Stephanie A.;Clay, Sharon A.
通讯作者：
Clay, Sharon A.