Histone H4R3 methylation catalyzed by SKB1/PRMT5 is required for maintaining shoot apical meristem.
Histone H4R3 methylation catalyzed by SKB1/PRMT5 is required for maintaining shoot apical meristem.
复制标题
DOI：
10.1371/journal.pone.0083258
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Bao S
中科院分区：
文献类型：
--
作者：
Yue M;Li Q;Zhang Y;Zhao Y;Zhang Z;Bao S
茎顶端分生组织（SAM）是高等植物胚后发育过程中所有地上组织和器官的来源。研究证明，构成WUSCHEL（WUS）-CLAVATA（CLV）反馈环的基因的表达对于SAM的维持至关重要。组蛋白赖氨酸乙酰化和甲基化标记已被证实调控WUS的转录水平。然而，关于组蛋白精氨酸甲基化如何调节WUS和其他基因的表达知之甚少。在这里，我们报告了由SKB 1/PRMT 5介导的H4 R3对称二甲基化（H4 R3 sme 2）抑制CORYNE（CRN）的表达，以维持正常的SAM几何形状。SKB 1损伤导致拟南芥中SAM尺寸变小，以及WUS和CLV 3的表达下调。上调WUS表达扩大了skb 1突变体植物中SAM的大小。我们发现SKB 1和H4 R3 sme 2与CRN基因座的染色质结合，下调其转录。CRN的突变拯救了WUS的表达和skb 1的小SAM尺寸。因此，SKB 1和SKB 1介导的H4 R3 sme 2是拟南芥幼苗中SAM维持所必需的。
The shoot apical meristem (SAM) is the source of all of the above-ground tissues and organs in post-embryonic development in higher plants. Studies have proven that the expression of genes constituting the WUSCHEL (WUS)-CLAVATA (CLV) feedback loop is critical for the SAM maintenance. Several histone lysine acetylation and methylation markers have been proven to regulate the transcription level of WUS. However, little is known about how histone arginine methylation regulates the expression of WUS and other genes. Here, we report that H4R3 symmetric dimethylation (H4R3sme2) mediated by SKB1/PRMT5 represses the expression of CORYNE (CRN) to maintain normal SAM geometrics. SKB1 lesion results in small SAM size in Arabidopsis, as well as down-regulated expression of WUS and CLV3. Up-regulation of WUS expression enlarges SAM size in skb1 mutant plants. We find that SKB1 and H4R3sme2 associate with the chromatin of the CRN locus to down-regulate its transcription. Mutation of CRN rescues the expression of WUS and the small SAM size of skb1. Thus, SKB1 and SKB1-mediated H4R3sme2 are required for the maintenance of SAM in Arabidopsis seedlings.
登录
查看更多内容
影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
4.9
作者：
Betsuyaku S;Takahashi F;Kinoshita A;Miwa H;Shinozaki K;Fukuda H;Sawa S
通讯作者：
Sawa S
影响因子：
7.4
作者：
Bleckmann, Andrea;Weidtkamp-Peters, Stefanie;Simon, Ruediger
通讯作者：
Simon, Ruediger
DOI：
10.1073/pnas.95.25.14781
发表时间：
1998-12-08
影响因子：
11.1
作者：
Gilbreth, M;Yang, PR;Marcus, S
通讯作者：
Marcus, S
影响因子：
7.2
作者：
Bowler, C;Benvenuto, G;Paszkowski, J
通讯作者：
Paszkowski, J