Postweaning exposure to a high-fat diet is associated with alterations to the hepatic histone code in Japanese macaques.
Postweaning exposure to a high-fat diet is associated with alterations to the hepatic histone code in Japanese macaques.
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DOI：
10.1038/pr.2013.106
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发表时间：
2013-09
影响因子：
3.6
通讯作者：
Aagaard, Kjersti M.
中科院分区：
文献类型：
--
作者：
Suter, Melissa A.;Takahashi, Diana;Grove, Kevin L.;Aagaard, Kjersti M.
由于胎儿组蛋白密码的改变，昼夜节律基因 Npas2 的表达在母亲高脂肪饮食暴露的胎儿生命中发生改变。我们假设这些破坏将在出生后持续存在。怀孕的猕猴被喂食对照（CTR）或高脂肪（HF）饮食并足月分娩。当后代断奶时，将它们置于 CTR 或 HF 饮食中 5 个月，以产生四种暴露模型（子宫内饮食/断奶后饮食：CTR/CTR n = 5；CTR/HF n = 4；HF/CTR n = 4；HF/HF n = 5）。肝脏标本是在一岁时尸检时获得的。组蛋白 H3 赖氨酸 4 的肝脏三甲基化降低（CTR/HF 0.87 倍，P = 0.038；HF/CTR 0.84 倍，P = 0.038），而肝脏甲基转移酶活性因饮食暴露而增加（HF/HF 1.3 倍，P = 0.019）。使用染色质免疫沉淀法来确定 Npas2 启动子的占据情况，我们发现抑制性和许可性组蛋白修饰都发生了变化，特别是在断奶后高脂肪饮食暴露的情况下。我们发现 Npas2 表达的改变与 Npas2 启动子内组蛋白代码的变化相对应。
Expression of the circadian gene, Npas2, is altered in fetal life with maternal high fat diet exposure by virtue of alterations in the fetal histone code. We postulated that these disruptions would persist postnatally. Pregnant macaques were fed a control (CTR) or high fat (HF) diet and delivered at term. When offspring were weaned, they were placed on either CTR or HF diet for a period of 5 months to yield four exposure models (in utero diet/postweaning diet: CTR/CTR n = 5; CTR/HF n = 4; HF/CTR n = 4; HF/HF n = 5). Liver specimens were obtained at necropsy at one year of age. Hepatic trimethylation of lysine 4 of histone H3 is decreased, (CTR/HF 0.87-fold, P = 0.038; HF/CTR 0.84-fold, P = 0.038) while hepatic methyltransferase activity increased by virtue of diet exposure (HF/HF 1.3-fold, P = 0.019). Using chromatin immunoprecipitation to determine Npas2 promoter occupancy, we found alterations of both repressive and permissive histone modifications specifically with postweaning high fat diet exposure. We find altered Npas2 expression corresponds with a change in the histone code within the Npas2 promoter.
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影响因子：
9.8
作者：
Cox, James;Williams, Sarah;Grove, Kevin;Lane, Robert H.;Aagaard-Tillery, Kjersti M.
通讯作者：
Aagaard-Tillery, Kjersti M.
影响因子：
10.9
作者：
Pinney, Sara E.;Simmons, Rebecca A.
通讯作者：
Simmons, Rebecca A.
DOI：
10.1523/jneurosci.5560-09.2010
发表时间：
2010-03-10
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Sullivan EL;Grayson B;Takahashi D;Robertson N;Maier A;Bethea CL;Smith MS;Coleman K;Grove KL
通讯作者：
Grove KL
影响因子：
56.9
作者：
Nakayam, J;Rice, JC;Grewal, SIS
通讯作者：
Grewal, SIS
影响因子：
--
作者：
Suter, Melissa A.;Sangi-Haghpeykar, Haleh;Aagaard, Kjersti M.
通讯作者：
Aagaard, Kjersti M.