Dynamics of quinolone resistance in fecal Escherichia coli of finishing pigs after ciprofloxacin administration.
Dynamics of quinolone resistance in fecal Escherichia coli of finishing pigs after ciprofloxacin administration.
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育肥猪施用环丙沙星后粪便大肠杆菌对喹诺酮类药物耐药性的动态变化
DOI：
10.1292/jvms.14-0025
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Wang HN
中科院分区：
文献类型：
--
作者：
Huang K;Xu CW;Zeng B;Xia QQ;Zhang AY;Lei CW;Guan ZB;Cheng H;Wang HN
目前，大肠杆菌对喹诺酮类药物的耐药性已成为中国大型养猪场的一个严重问题。因此，有必要研究猪粪大肠埃希菌对喹诺酮类药物的耐药性动态。在这里，我们提出一个假设，即猪体内出现耐药性需要药物积累7天或更长时间。为验证这一假设，选取断奶后未饲喂任何抗微生物药物的90日龄、约30 kg猪26头，分为2个组：试验组（EP）和对照组（CP）。EP组猪每天口服5 mg /kg体重的环丙沙星，连续30 d； CP组猪饲喂正常饲粮。在第0 ~ 61天的16个时间点收集新鲜粪便。在每个时间点，检测10个大肠杆菌克隆对喹诺酮类药物的敏感性以及gyrA和parC的突变。结果表明，环丙沙星最小抑制浓度（MIC）在给药3天后较初始MIC（0.5µg/ml）提高16倍，停药26天后较初始MIC（0.5µg/ml）降低256倍。所有MIC≥8µg/ml的耐喹诺酮大肠杆菌（QREC）克隆均出现GyrA （S83L、D87N/ D87Y）和parC （S80I）取代。本研究为抗菌药物的合理使用和细菌耐药性的控制提供了科学的理论指导。
Escherichia coli resistance to quinolones has now become a serious issue in large-scale pig farms of China. It is necessary to study the dynamics of quinolone resistance in fecal Escherichia coli of pigs after antimicrobial administration. Here, we present the hypothesis that the emergence of resistance in pigs requires drug accumulation for 7 days or more. To test this hypothesis, 26 pigs (90 days old, about 30 kg) not fed any antimicrobial after weaning were selected and divided into 2 equal groups: the experimental (EP) group and control (CP) group. Pigs in the EP group were orally treated daily with 5 mg ciprofloxacin/kg of body weight for 30 days, and pigs in the CP group were fed a normal diet. Fresh feces were collected at 16 time points from day 0 to day 61. At each time point, ten E. coli clones were tested for susceptibility to quinolones and mutations of gyrA and parC. The results showed that the minimal inhibitory concentration (MIC) for ciprofloxacin increased 16-fold compared with the initial MIC (0.5 µg/ml) after ciprofloxacin administration for 3 days and decreased 256-fold compared with the initial MIC (0.5 µg/ml) after ciprofloxacin withdrawal for 26 days. GyrA (S83L, D87N/ D87Y) and parC (S80I) substitutions were observed in all quinolone-resistant E. coli (QREC) clones with an MIC ≥8 µg/ml. This study provides scientific theoretical guidance for the rational use of antimicrobials and the control of bacterial resistance.
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影响因子：
3.3
作者：
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