Case-control study of microbiological etiology associated with calf diarrhea.
Case-control study of microbiological etiology associated with calf diarrhea.
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DOI：
10.1016/j.vetmic.2013.07.001
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发表时间：
2013-10-25
影响因子：
3.3
通讯作者：
Yoon KJ
中科院分区：
文献类型：
--
作者：
Cho YI;Han JI;Wang C;Cooper V;Schwartz K;Engelken T;Yoon KJ
小牛腹泻是美国养牛业的主要经济负担。犊牛腹泻涉及多种传染性病原体，多种病原体的合并感染在腹泻犊牛中并不少见。进行了一项病例对照研究，以评估与中西部牛场小牛腹泻相关的感染性病因。从165个牛场分别采集了199和245份健康小牛粪便样品。通过一组多重PCR检测试剂盒检测样品中的11种肠道病原体：牛轮状病毒A组（BRV-A）、牛冠状病毒（BCoV）、牛病毒性腹泻病毒（BVDV）、牛肠道病毒（BEV）、牛诺如病毒（BNoV）、Nebovirus、牛圆环病毒（BToV）、沙门氏菌属。（沙门氏菌）、大肠杆菌（E. coli）K99+、带β毒素基因的产气荚膜梭菌和隐孢子虫（C. parvum）。腹泻和每种病原体的检测之间的关联使用多变量逻辑回归模型进行分析。超过一半的腹泻小牛粪便样本有多种病原体。BRV-A、BCoV、BNoV、Nebovirus、Salmonella、E. coliK 99+和C.犊牛腹泻与仔猪腹泻显著相关（p < 0.05）。其中C.细小病毒和BRV-A被认为是小牛腹泻最常见的肠道病原体，检测频率高（33.7%和27.1%），优势比高（173和79.9）。出乎意料的是，BNoV（OR = 2.0）和Nebovirus（OR = 16.7）在腹泻小牛中的频率很高，表明这些病毒可能对小牛腹泻有显著影响。
Calf diarrhea is a major economic burden for the US cattle industry. A variety of infectious agents are implicated in calf diarrhea and co-infection of multiple pathogens is not uncommon in diarrheic calves. A case–control study was conducted to assess infectious etiologies associated with calf diarrhea in Midwest cattle farms. A total of 199 and 245 fecal samples were obtained from diarrheic and healthy calves, respectively, from 165 cattle farms. Samples were tested by a panel of multiplex PCR assays for 11 enteric pathogens: bovine rotavirus group A (BRV-A), bovine coronavirus (BCoV), bovine viral diarrhea virus (BVDV), bovine enterovirus (BEV), bovine norovirus (BNoV), Nebovirus, bovine torovirus (BToV) Salmonella spp. (Salmonella), Escherichia coli (E. coli) K99+, Clostridium perfringens with β toxin gene and Cryptosporidium parvum (C. parvum). The association between diarrhea and detection of each pathogen was analyzed using a multivariate logistic regression model. More than a half of the fecal samples from the diarrheic calves had multiple pathogens. Statistically, BRV-A, BCoV, BNoV, Nebovirus, Salmonella, E. coli K99+, and C. parvum were significantly associated with calf diarrhea (p < 0.05). Among them, C. parvum and BRV-A were considered to be the most common enteric pathogens for calf diarrhea with high detection frequency (33.7% and 27.1%) and strong odds ratio (173 and 79.9). Unexpectedly BNoV (OR = 2.0) and Nebovirus (OR = 16.7) were identified with high frequency in diarrheic calves, suggesting these viruses may have a significant contribution to calf diarrhea.
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