Glutamate oxidase biosensor based on mixed ceria and titania nanoparticles for the detection of glutamate in hypoxic environments.
Glutamate oxidase biosensor based on mixed ceria and titania nanoparticles for the detection of glutamate in hypoxic environments.
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DOI：
10.1016/j.bios.2013.08.054
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发表时间：
2014-02-15
影响因子：
12.6
通讯作者：
Andreescu, Silvana
中科院分区：
文献类型：
--
作者：
Oezel, Rifat Emrah;Ispas, Cristina;Ganesana, Mallikarjunarao;Leiter, J. C.;Andreescu, Silvana
我们报告的设计和开发的谷氨酸氧化酶（GmOx）微电极测量L-谷氨酸（GluA）在缺氧条件下，这是基于氧化铈的储氧和释氧能力。为了制造的生物传感器，富氧的二氧化铈和二氧化钛纳米粒子分散在半渗透壳聚糖膜的纳米复合材料与酶GmOx的Pt微电极的表面上共同固定。包埋在生物相容性壳聚糖基质中的二氧化铈纳米颗粒的氧递送能力促进了酶在无氧条件下的稳定和操作。GluA通过电流分析法在相对于Ag/AgCl为0.6V的工作电位下测量。在氧化和去氧条件下，检测限分别为0.594 μM和0.493 μM，灵敏度分别为793 pA/μM（RSD 3.49%，n=5）和395 pA/μM（RSD 2.48%，n=5），响应时间分别为2s和5s。该传感器对常见干扰物质具有良好的操作稳定性和选择性。在脑脊液中测试了生物传感器的操作。在Sprague-Dawley大鼠脑缺血和再灌注期间监测皮层中GluA的初步体内记录表明了生物传感器在缺氧条件下的潜在应用。该方法提供了确保氧化还原酶在无氧环境中的功能性的解决方案。
We report on the design and development of a glutamate oxidase (GmOx) microelectrode for measuring L-glutamic acid (GluA) in oxygen-depleted conditions, which is based on the oxygen storage and release capacity of cerium oxides. To fabricate the biosensor, a nanocomposite of oxygen-rich ceria and titania nanoparticles dispersed within a semi-permeable chitosan membrane was co-immobilized with the enzyme GmOx on the surface of a Pt microelectrode. The oxygen delivery capacity of the ceria nanoparticles embedded in a biocompatible chitosan matrix facilitated enzyme stabilization and operation in oxygen free conditions. GluA was measured by amperometry at a working potential of 0.6 V vs Ag/AgCl. Detection limits of 0.594 μM and 0.493 μM and a sensitivity of 793 pA/μM (RSD 3.49%, n=5) and 395 pA/μM (RSD 2.48%, n=5) were recorded in oxygenated and deoxygenated conditions, with response times of 2s and 5s, respectively. The biosensor had good operational stability and selectivity against common interfering substances. Operation of the biosensor was tested in cerebrospinal fluid. Preliminary in vivo recording in Sprague-Dawley rats to monitor GluA in the cortex during cerebral ischemia and reperfusion demonstrate a potential application of the biosensor in hypoxic conditions. This method provides a solution to ensure functionality of oxidoreductase enzymes in oxygen-free environments.
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影响因子：
7.4
作者：
Ornatska, Maryna;Sharpe, Erica;Andreescu, Silvana
通讯作者：
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影响因子：
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作者：
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